In vitro metal uptake by recombinant human manganese superoxide dismutase

Mei M. Whittaker, James Whittaker

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Metal uptake by the antioxidant defense metalloenzyme manganese superoxide dismutase (MnSOD) is an essential step in the functional maturation of the protein that is just beginning to be investigated in detail. We have extended earlier in vitro studies on metal binding by the dimeric Escherichia coli apo-MnSOD to investigate the mechanism of metal uptake by tetrameric human and Thermus thermophilus apo-MnSODs. Like the E. coli apo-MnSOD, these proteins also bind metal ions in vitro in a thermally activated, pH-sensitive process. However, metal uptake by the tetrameric apo-MnSODs exhibits a number of important differences. In particular, there is no indication of conformational gating requirement for metal binding for these proteins, and the reaction is first-order in metal ion. The high concentration of metal ion that is required to achieve physiologically relevant metallation rates for tetrameric human apo-MnSOD in vitro suggests the possibility that co-translational metal binding or chaperone interactions may be required in vivo.

Original languageEnglish (US)
Pages (from-to)69-74
Number of pages6
JournalArchives of Biochemistry and Biophysics
Volume491
Issue number1-2
DOIs
StatePublished - Nov 2009
Externally publishedYes

Fingerprint

Superoxide Dismutase
Metals
Metal ions
Escherichia coli
Ions
Thermus thermophilus
In Vitro Techniques
Carrier Proteins
Proteins
Antioxidants

Keywords

  • Antioxidant
  • Human manganese superoxide dismutase
  • Mechanism
  • Metal uptake

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

In vitro metal uptake by recombinant human manganese superoxide dismutase. / Whittaker, Mei M.; Whittaker, James.

In: Archives of Biochemistry and Biophysics, Vol. 491, No. 1-2, 11.2009, p. 69-74.

Research output: Contribution to journalArticle

Whittaker, Mei M. ; Whittaker, James. / In vitro metal uptake by recombinant human manganese superoxide dismutase. In: Archives of Biochemistry and Biophysics. 2009 ; Vol. 491, No. 1-2. pp. 69-74.
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