In vitro immunogenicity of trinitrophenylated bacteriophage T4. I. Lack of helper cell cooperation

J. J. Jennings, M. Baltz, M. B. Rittenberg

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8 Scopus citations


A primary immune response was obtained in vitro to trinitrophenylated bacteriophage T4 (TNP T4) by using normal BALB/c spleen cells challenged in vitro. The primary response was limited to anti TNP; we failed to find evidence of a primary anti T4 response in spleen cell cultures challenged with either TNP T4 or native T4. In vivo priming with the carrier T4 failed to enhance the subsequent in vitro response to TNP T4 although such priming sometimes caused suppression of the anti hapten response. The addition of excess free carrier to spleen cell cultures challenged with TNP T4 failed to suppress the anti TNP response. Spleen cells treated with anti theta plus complement were unaffected in their ability to respond to TNP T4. It is concluded that TNP T4 should be classified among the thymus independent antigens.

Original languageEnglish (US)
Pages (from-to)1432-1437
Number of pages6
JournalJournal of Immunology
Issue number5
StatePublished - Dec 1 1975


ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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