In vitro immune absorption of antisperm antibodies with immunobead-rise, immunomagnetic, and immunocolumn separation techniques

G. C. Kiser, N. J. Alexander, Eugene Fuchs, D. L. Fulgham

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

Fourteen men with a mean duration of infertility >3 years who had significant sperm immobilizing or sperm-agglutinating antibodies were studied. All patients had >20% IgG or IgA immunobinding to sperm in their seminal plasma and 7 had immunobinding levels of >50%. Sperm from these men were less able to penetrate an overlaying buffer layer than sperm from a fertile control. Addition of immunobeads to the specimen was of little use, because few motile sperm could swim into the overlaying buffer; retained immunobeads were noted in the buffer layer of 18-hour capacitated specimens. Magnetic isolation of antibody-coated sperm from antibody-free sperm avoids potential damage to fragile sperm through centrifugation. Viable spermatozoa were isolated from magnetite-complexed spermatozoa, but the motility of the isolated spermatozoa deteriorated rapidly during the subsequent capacitation period. Passage of diluted ejaculate through a column of dextran beads for antisperm antibody processing (ASAP) was associated with superior sperm quality and fertilizing potential. The use of ASAP resulted in good sperm velocity and linearity and improved sperm function, as measured with the hamster egg penetration test. Sperm from men with immunologically mediated infertility can be processed through the ASAP and used for artificial insemination of their partners or in an in vitro fertilization program.

Original languageEnglish (US)
Pages (from-to)466-474
Number of pages9
JournalFertility and Sterility
Volume47
Issue number3
StatePublished - 1987

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Immunomagnetic Separation
Spermatozoa
Antibodies
Buffers
In Vitro Techniques
Infertility
Ferrosoferric Oxide
Artificial Insemination

ASJC Scopus subject areas

  • Obstetrics and Gynecology

Cite this

In vitro immune absorption of antisperm antibodies with immunobead-rise, immunomagnetic, and immunocolumn separation techniques. / Kiser, G. C.; Alexander, N. J.; Fuchs, Eugene; Fulgham, D. L.

In: Fertility and Sterility, Vol. 47, No. 3, 1987, p. 466-474.

Research output: Contribution to journalArticle

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