In Vitro Culture of Rhesus Macaque (Macaca mulatta) Embryos

Cathy Ramsey, Carol Hanna

    Research output: Chapter in Book/Report/Conference proceedingChapter

    Abstract

    Production of nonhuman primate (NHP) embryos in vitro begins with recovery of gametes. Females undergo a controlled ovary stimulation to produce multiple preovulatory follicles from which oocytes may be recovered. Mature ova are subjected to in vitro fertilization (IVF) and presumptive zygotes are cultured to the intended stage of development. Essential to this practice is the culture medium unique to each step in the process. Here we describe medium preparation, oocyte recovery, in vitro fertilization, and in vitro culture (IVC) of embryos in the rhesus macaque model.

    Original languageEnglish (US)
    Title of host publicationMethods in Molecular Biology
    PublisherHumana Press Inc.
    Pages341-353
    Number of pages13
    DOIs
    StatePublished - Jan 1 2019

    Publication series

    NameMethods in Molecular Biology
    Volume2006
    ISSN (Print)1064-3745

    Fingerprint

    Fertilization in Vitro
    Macaca mulatta
    Embryonic Structures
    Oocytes
    Zygote
    Germ Cells
    Primates
    Ovum
    Culture Media
    Ovary
    In Vitro Techniques

    Keywords

    • Controlled ovarian stimulation
    • Embryo culture
    • Fertilization
    • Nonhuman primate
    • Rhesus macaque

    ASJC Scopus subject areas

    • Molecular Biology
    • Genetics

    Cite this

    Ramsey, C., & Hanna, C. (2019). In Vitro Culture of Rhesus Macaque (Macaca mulatta) Embryos. In Methods in Molecular Biology (pp. 341-353). (Methods in Molecular Biology; Vol. 2006). Humana Press Inc.. https://doi.org/10.1007/978-1-4939-9566-0_23

    In Vitro Culture of Rhesus Macaque (Macaca mulatta) Embryos. / Ramsey, Cathy; Hanna, Carol.

    Methods in Molecular Biology. Humana Press Inc., 2019. p. 341-353 (Methods in Molecular Biology; Vol. 2006).

    Research output: Chapter in Book/Report/Conference proceedingChapter

    Ramsey, C & Hanna, C 2019, In Vitro Culture of Rhesus Macaque (Macaca mulatta) Embryos. in Methods in Molecular Biology. Methods in Molecular Biology, vol. 2006, Humana Press Inc., pp. 341-353. https://doi.org/10.1007/978-1-4939-9566-0_23
    Ramsey C, Hanna C. In Vitro Culture of Rhesus Macaque (Macaca mulatta) Embryos. In Methods in Molecular Biology. Humana Press Inc. 2019. p. 341-353. (Methods in Molecular Biology). https://doi.org/10.1007/978-1-4939-9566-0_23
    Ramsey, Cathy ; Hanna, Carol. / In Vitro Culture of Rhesus Macaque (Macaca mulatta) Embryos. Methods in Molecular Biology. Humana Press Inc., 2019. pp. 341-353 (Methods in Molecular Biology).
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