In Vitro Culture of Rhesus Macaque (Macaca mulatta) Embryos

Cathy Ramsey; Carol Hanna

doi:10.1007/978-1-4939-9566-0_23

In Vitro Culture of Rhesus Macaque (Macaca mulatta) Embryos

Cathy Ramsey, Carol Hanna

Research output: Chapter in Book/Report/Conference proceeding › Chapter

2 Scopus citations

Abstract

Production of nonhuman primate (NHP) embryos in vitro begins with recovery of gametes. Females undergo a controlled ovary stimulation to produce multiple preovulatory follicles from which oocytes may be recovered. Mature ova are subjected to in vitro fertilization (IVF) and presumptive zygotes are cultured to the intended stage of development. Essential to this practice is the culture medium unique to each step in the process. Here we describe medium preparation, oocyte recovery, in vitro fertilization, and in vitro culture (IVC) of embryos in the rhesus macaque model.

Original language	English (US)
Title of host publication	Methods in Molecular Biology
Publisher	Humana Press Inc.
Pages	341-353
Number of pages	13
DOIs	https://doi.org/10.1007/978-1-4939-9566-0_23
State	Published - 2019

Publication series

Name	Methods in Molecular Biology
Volume	2006
ISSN (Print)	1064-3745
ISSN (Electronic)	1940-6029

Keywords

Controlled ovarian stimulation
Embryo culture
Fertilization
Nonhuman primate
Rhesus macaque

ASJC Scopus subject areas

Molecular Biology
Genetics

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10.1007/978-1-4939-9566-0_23

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In Vitro Culture of Rhesus Macaque (Macaca mulatta) Embryos

Abstract

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Keywords

ASJC Scopus subject areas

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