Abstract
Production of nonhuman primate (NHP) embryos in vitro begins with recovery of gametes. Females undergo a controlled ovary stimulation to produce multiple preovulatory follicles from which oocytes may be recovered. Mature ova are subjected to in vitro fertilization (IVF) and presumptive zygotes are cultured to the intended stage of development. Essential to this practice is the culture medium unique to each step in the process. Here we describe medium preparation, oocyte recovery, in vitro fertilization, and in vitro culture (IVC) of embryos in the rhesus macaque model.
| Original language | English (US) |
|---|---|
| Title of host publication | Methods in Molecular Biology |
| Publisher | Humana Press Inc. |
| Pages | 341-353 |
| Number of pages | 13 |
| DOIs | |
| State | Published - Jan 1 2019 |
Publication series
| Name | Methods in Molecular Biology |
|---|---|
| Volume | 2006 |
| ISSN (Print) | 1064-3745 |
Keywords
- Controlled ovarian stimulation
- Embryo culture
- Fertilization
- Nonhuman primate
- Rhesus macaque
ASJC Scopus subject areas
- Molecular Biology
- Genetics
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Cite this
Ramsey, C., & Hanna, C. (2019). In Vitro Culture of Rhesus Macaque (Macaca mulatta) Embryos. In Methods in Molecular Biology (pp. 341-353). (Methods in Molecular Biology; Vol. 2006). Humana Press Inc.. https://doi.org/10.1007/978-1-4939-9566-0_23