@inbook{7a3e7894b92a42b9ac63c84db81b904c,
title = "In Vitro Culture of Rhesus Macaque (Macaca mulatta) Embryos",
abstract = "Production of nonhuman primate (NHP) embryos in vitro begins with recovery of gametes. Females undergo a controlled ovary stimulation to produce multiple preovulatory follicles from which oocytes may be recovered. Mature ova are subjected to in vitro fertilization (IVF) and presumptive zygotes are cultured to the intended stage of development. Essential to this practice is the culture medium unique to each step in the process. Here we describe medium preparation, oocyte recovery, in vitro fertilization, and in vitro culture (IVC) of embryos in the rhesus macaque model.",
keywords = "Controlled ovarian stimulation, Embryo culture, Fertilization, Nonhuman primate, Rhesus macaque",
author = "Cathy Ramsey and Carol Hanna",
note = "Publisher Copyright: {\textcopyright} Springer Science+Business Media, LLC, part of Springer Nature 2019.",
year = "2019",
doi = "10.1007/978-1-4939-9566-0_23",
language = "English (US)",
series = "Methods in Molecular Biology",
publisher = "Humana Press Inc.",
pages = "341--353",
booktitle = "Methods in Molecular Biology",
}