In Vitro Culture of Rhesus Macaque (Macaca mulatta) Embryos

Cathy Ramsey, Carol Hanna

    Research output: Chapter in Book/Report/Conference proceedingChapter

    2 Scopus citations

    Abstract

    Production of nonhuman primate (NHP) embryos in vitro begins with recovery of gametes. Females undergo a controlled ovary stimulation to produce multiple preovulatory follicles from which oocytes may be recovered. Mature ova are subjected to in vitro fertilization (IVF) and presumptive zygotes are cultured to the intended stage of development. Essential to this practice is the culture medium unique to each step in the process. Here we describe medium preparation, oocyte recovery, in vitro fertilization, and in vitro culture (IVC) of embryos in the rhesus macaque model.

    Original languageEnglish (US)
    Title of host publicationMethods in Molecular Biology
    PublisherHumana Press Inc.
    Pages341-353
    Number of pages13
    DOIs
    StatePublished - 2019

    Publication series

    NameMethods in Molecular Biology
    Volume2006
    ISSN (Print)1064-3745
    ISSN (Electronic)1940-6029

    Keywords

    • Controlled ovarian stimulation
    • Embryo culture
    • Fertilization
    • Nonhuman primate
    • Rhesus macaque

    ASJC Scopus subject areas

    • Molecular Biology
    • Genetics

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