In vitro biofilm formation on resin-based composites cured under different surface conditions

Andrei C. Ionescu, Gloria Cazzaniga, Marco Ottobelli, Jack Ferracane, Gaetano Paolone, Eugenio Brambilla

Research output: Contribution to journalArticle

Abstract

Objectives: The interfacial conditions occurring during light-curing procedures of resin-based composites (RBCs) influence their surface properties and therefore the biological behavior of the material. This study aimed to evaluate the influence of different surface curing conditions on in vitro biofilm formation by Streptococcus mutans and mixed oral microflora, in the presence or absence of surface salivary pre-conditioning. Methods: Two nanohybrid RBCs and four interfacial curing conditions (open air, argon, nitrogen and glycerin) were evaluated. Surface roughness (SR), surface elemental composition (energy-dispersive X-ray spectrometry, EDS) and biofilm formation (S. mutans and oral microcosm) were assessed. Surfaces were observed using scanning electron microscopy (SEM). Microbiological tests were performed with and without saliva pre-conditioning of the surfaces. EDS analysis was performed before and after biofilm formation, and biofilm morphology was evaluated using confocal laser scanning microscopy (CLSM). Data were analyzed using multi-way ANOVA and Tukey post-hoc test (p < 0.05). Results: Interfacial curing conditions significantly influenced SR depending on the tested RBC. EDS analysis showed that surface elemental composition was significantly influenced by the interfacial curing condition depending on the tested RBC. Interfacial curing conditions significantly influenced biofilm formation in both microbiological models in the absence of saliva pre-conditioning, depending on the tested RBC, whereas saliva pre-conditioning abrogated these effects. Conclusions: Surface curing conditions significantly impacted biofilm formation in a material-dependent manner, which was abrogated when surfaces were pre-conditioned with saliva. Clinical significance: Curing under glycerin did not improve the microbiological performances of the tested RBCs. These results, needing to be confirmed by in vivo data, have the potential to simplify operative procedures in restorative dentistry.

Original languageEnglish (US)
JournalJournal of Dentistry
DOIs
StateAccepted/In press - Jan 1 2018

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Composite Resins
Biofilms
Saliva
Streptococcus mutans
Glycerol
X-Ray Emission Spectrometry
Surface Properties
Argon
Operative Surgical Procedures
Dentistry
Confocal Microscopy
Electron Scanning Microscopy
In Vitro Techniques
Analysis of Variance
Nitrogen
Air
Light

Keywords

  • Biofilm
  • Bioreactor
  • Light-curing
  • Resin-based composites
  • Salivary pellicle
  • Streptococcus mutans

ASJC Scopus subject areas

  • Dentistry(all)

Cite this

In vitro biofilm formation on resin-based composites cured under different surface conditions. / Ionescu, Andrei C.; Cazzaniga, Gloria; Ottobelli, Marco; Ferracane, Jack; Paolone, Gaetano; Brambilla, Eugenio.

In: Journal of Dentistry, 01.01.2018.

Research output: Contribution to journalArticle

Ionescu, Andrei C. ; Cazzaniga, Gloria ; Ottobelli, Marco ; Ferracane, Jack ; Paolone, Gaetano ; Brambilla, Eugenio. / In vitro biofilm formation on resin-based composites cured under different surface conditions. In: Journal of Dentistry. 2018.
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title = "In vitro biofilm formation on resin-based composites cured under different surface conditions",
abstract = "Objectives: The interfacial conditions occurring during light-curing procedures of resin-based composites (RBCs) influence their surface properties and therefore the biological behavior of the material. This study aimed to evaluate the influence of different surface curing conditions on in vitro biofilm formation by Streptococcus mutans and mixed oral microflora, in the presence or absence of surface salivary pre-conditioning. Methods: Two nanohybrid RBCs and four interfacial curing conditions (open air, argon, nitrogen and glycerin) were evaluated. Surface roughness (SR), surface elemental composition (energy-dispersive X-ray spectrometry, EDS) and biofilm formation (S. mutans and oral microcosm) were assessed. Surfaces were observed using scanning electron microscopy (SEM). Microbiological tests were performed with and without saliva pre-conditioning of the surfaces. EDS analysis was performed before and after biofilm formation, and biofilm morphology was evaluated using confocal laser scanning microscopy (CLSM). Data were analyzed using multi-way ANOVA and Tukey post-hoc test (p < 0.05). Results: Interfacial curing conditions significantly influenced SR depending on the tested RBC. EDS analysis showed that surface elemental composition was significantly influenced by the interfacial curing condition depending on the tested RBC. Interfacial curing conditions significantly influenced biofilm formation in both microbiological models in the absence of saliva pre-conditioning, depending on the tested RBC, whereas saliva pre-conditioning abrogated these effects. Conclusions: Surface curing conditions significantly impacted biofilm formation in a material-dependent manner, which was abrogated when surfaces were pre-conditioned with saliva. Clinical significance: Curing under glycerin did not improve the microbiological performances of the tested RBCs. These results, needing to be confirmed by in vivo data, have the potential to simplify operative procedures in restorative dentistry.",
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AU - Ionescu, Andrei C.

AU - Cazzaniga, Gloria

AU - Ottobelli, Marco

AU - Ferracane, Jack

AU - Paolone, Gaetano

AU - Brambilla, Eugenio

PY - 2018/1/1

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N2 - Objectives: The interfacial conditions occurring during light-curing procedures of resin-based composites (RBCs) influence their surface properties and therefore the biological behavior of the material. This study aimed to evaluate the influence of different surface curing conditions on in vitro biofilm formation by Streptococcus mutans and mixed oral microflora, in the presence or absence of surface salivary pre-conditioning. Methods: Two nanohybrid RBCs and four interfacial curing conditions (open air, argon, nitrogen and glycerin) were evaluated. Surface roughness (SR), surface elemental composition (energy-dispersive X-ray spectrometry, EDS) and biofilm formation (S. mutans and oral microcosm) were assessed. Surfaces were observed using scanning electron microscopy (SEM). Microbiological tests were performed with and without saliva pre-conditioning of the surfaces. EDS analysis was performed before and after biofilm formation, and biofilm morphology was evaluated using confocal laser scanning microscopy (CLSM). Data were analyzed using multi-way ANOVA and Tukey post-hoc test (p < 0.05). Results: Interfacial curing conditions significantly influenced SR depending on the tested RBC. EDS analysis showed that surface elemental composition was significantly influenced by the interfacial curing condition depending on the tested RBC. Interfacial curing conditions significantly influenced biofilm formation in both microbiological models in the absence of saliva pre-conditioning, depending on the tested RBC, whereas saliva pre-conditioning abrogated these effects. Conclusions: Surface curing conditions significantly impacted biofilm formation in a material-dependent manner, which was abrogated when surfaces were pre-conditioned with saliva. Clinical significance: Curing under glycerin did not improve the microbiological performances of the tested RBCs. These results, needing to be confirmed by in vivo data, have the potential to simplify operative procedures in restorative dentistry.

AB - Objectives: The interfacial conditions occurring during light-curing procedures of resin-based composites (RBCs) influence their surface properties and therefore the biological behavior of the material. This study aimed to evaluate the influence of different surface curing conditions on in vitro biofilm formation by Streptococcus mutans and mixed oral microflora, in the presence or absence of surface salivary pre-conditioning. Methods: Two nanohybrid RBCs and four interfacial curing conditions (open air, argon, nitrogen and glycerin) were evaluated. Surface roughness (SR), surface elemental composition (energy-dispersive X-ray spectrometry, EDS) and biofilm formation (S. mutans and oral microcosm) were assessed. Surfaces were observed using scanning electron microscopy (SEM). Microbiological tests were performed with and without saliva pre-conditioning of the surfaces. EDS analysis was performed before and after biofilm formation, and biofilm morphology was evaluated using confocal laser scanning microscopy (CLSM). Data were analyzed using multi-way ANOVA and Tukey post-hoc test (p < 0.05). Results: Interfacial curing conditions significantly influenced SR depending on the tested RBC. EDS analysis showed that surface elemental composition was significantly influenced by the interfacial curing condition depending on the tested RBC. Interfacial curing conditions significantly influenced biofilm formation in both microbiological models in the absence of saliva pre-conditioning, depending on the tested RBC, whereas saliva pre-conditioning abrogated these effects. Conclusions: Surface curing conditions significantly impacted biofilm formation in a material-dependent manner, which was abrogated when surfaces were pre-conditioned with saliva. Clinical significance: Curing under glycerin did not improve the microbiological performances of the tested RBCs. These results, needing to be confirmed by in vivo data, have the potential to simplify operative procedures in restorative dentistry.

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KW - Bioreactor

KW - Light-curing

KW - Resin-based composites

KW - Salivary pellicle

KW - Streptococcus mutans

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