In vitro assembly of apophytochrome and apophytochrome deletion mutants expressed in yeast with phycocyanobilin

Lily Deforce, Ken Ichi Tomizawa, Naoko Ito, Dave Farrens, Pill Soon Song, Masaki Furuya

Research output: Contribution to journalArticlepeer-review

55 Scopus citations

Abstract

Recombinant pea type I phytochrome apoprotein expressed in yeast is shown to assemble in vitro with phycocyanobilin to produce a photoreversible phytochrome-like adduct. As an initial investigation of the amino acid sequence requirements for chromophore incorporation, three phyA gene product deletion mutants were produced in yeast. Truncation of the N-terminal tail to residue 46 demonstrates that this region is not critical to bilin attachment, but a deletion mutant lacking 222 amino acids from the N terminus failed to yield holophytochrome in vitro, under the same conditions. A mutant comprising a deletion of the C terminus to residue 548 showed bilin incorporation and red/far-red photoreversibility, indicating that bilin-apophytochrome assembly still occurred even when the entire C-terminal domain was truncated. (.

Original languageEnglish (US)
Pages (from-to)10392-10396
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume88
Issue number23
DOIs
StatePublished - 1991

Keywords

  • Phytochrome
  • Phytochrome biosynthesis
  • Plant photoreceptor
  • Recombinant

ASJC Scopus subject areas

  • General

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