Abstract
This study investigated the in vitro and in vivo antioxidant and anti-inflammatory properties of a juice blend (JB), MonaVie Active, containing a mixture of fruits and berries with known antioxidant activity, including açai, a palm fruit, as the predominant ingredient. The phytochemical antioxidants in the JB are primarily in the form of anthocyanins, predominantly cyanidin 3-rutoside, cyanidin 3-diglycoside, and cyanidin 3-glucoside. The cell-based antioxidant protection of erythrocytes (CAP-e) assay demonstrated that antioxidants in the JB penetrated and protected cells from oxidative damage (p < 0.001), whereas polymorphonuclear cells showed reduced formation of reactive oxygen species (p < 0.003) and reduced migration toward three different pro-inflammatory chemoattractants: fmlp (p < 0.001), leukotriene B4 (p < 0.05), and IL-8 (p < 0.03). A randomized, double-blinded, placebo-controlled, crossover trial with 12 healthy subjects examined the JB's antioxidant activity in vivo. Blood samples at baseline, 1 h, and 2 h following consumption of the JB or placebo were tested for antioxidant capacity using several antioxidant assays and the TBARS assay, a measure of lipid peroxidation. A within subject comparison showed an increase in serum antioxidants at 1 h (p < 0.03) and 2 h (p < 0.015), as well as inhibition of lipid peroxidation at 2 h (p < 0.01) postconsumption.
Original language | English (US) |
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Pages (from-to) | 8326-8333 |
Number of pages | 8 |
Journal | Journal of Agricultural and Food Chemistry |
Volume | 56 |
Issue number | 18 |
DOIs | |
State | Published - Sep 24 2008 |
Externally published | Yes |
Keywords
- Acai
- Anti-inflammatory
- Antioxidant
- Cell-based antioxidant protection assay (CAP-e)
- Euterpe oleracear
- Fruit juice
- Lipid peroxidation
- Oxygen radical absorbance capacity (ORAC) assay
- Thiobarbituric acid reactive substances assay (TBARS)
ASJC Scopus subject areas
- General Chemistry
- General Agricultural and Biological Sciences