In vitro and alternative approaches to developmental neurotoxicity

Lucio G. Costa, Claudia Pellacani, Marina Guizzetti

Research output: Chapter in Book/Report/Conference proceedingChapter

4 Scopus citations

Abstract

In recent years, the need to develop acceptable alternatives to conventional animal testing for neurotoxicity and developmental neurotoxicity has been increasingly recognized. Hence efforts are being directed toward the development of alternative models, utilizing either mammalian cells in culture or nonmammalian model systems. Such in vitro testing procedures have the purposes of investigating mode and/or mechanism of action of chemicals, particularly related to early, upstream events in the neurotoxic process and of screening chemicals of unknown toxicity to flag compounds for further in vitro and in vivo neurotoxicity studies. Several cell culture models exist, with different levels of complexity, and a major issue of debate remains that of the end points of toxicity to be measured. Screening for developmental neurotoxicity may require additional specific end points. Promising nonmammalian models include zebrafish and Caenorhabditis elegans. These issues are discussed in this chapter, together with considerations for further developments and validation of alternative models.

Original languageEnglish (US)
Title of host publicationReproductive and Developmental Toxicology
PublisherElsevier
Pages241-253
Number of pages13
ISBN (Electronic)9780128042397
DOIs
StatePublished - Jan 1 2017

Keywords

  • Alternative models
  • C. elegans
  • Cell differentiation
  • Cell proliferation
  • Cell viability
  • Developmental neurotoxicity
  • End-points of neurotoxicity
  • In vitro cell culture systems
  • Neurotoxicity
  • Validation
  • Zebrafish

ASJC Scopus subject areas

  • Medicine(all)

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