In Vitro Activation of Human Peripheral Blood Mononuclear Cells Induced by Human Biologic Meshes1

Sean Orenstein, Yi Qiao, Manjot Kaur, Ulrike Klueh, Don Kreutzer, Yuri Novitsky

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

Background: Inflammation and wound healing play critical roles in the integration of biologic meshes (BMs) at sites of hernia repair. Monocytes/macrophages (M/MQs) are key cells involved in mesh integration. Interleukin-1β (IL-1β) is one of the major M/MQ-derived cytokines, and its expression is a reflection of the degree of M/MQ activation. We hypothesized that BMs induce M/MQ activation in vitro and that IL-1β expression by M/MQ varies among various BMs. Materials and Methods: Acellular human dermis-derived BM samples (AlloDerm, AlloMax, FlexHD) were placed in 48-well plates and cultured with peripheral blood mononuclear cells (PBMCs) from three healthy human subjects for 7 d. The resulting supernatants were assayed for IL-1β levels by enzyme-linked immunosorbent assay (ELISA), and the BMs were evaluated histologically. Results: IL-1β expression varied among donors as well as the BMs [AlloDerm (2.11-38.25pg/106 PBMCs); AlloMax (13.12-715.40pg/106 PBMCs); and FlexHD (116.69-665.40pg/106 PBMCs)]. Analysis of this data indicated that AlloMax and FlexHD induced significantly more M/MQ activation compared with AlloDerm (P <0.05). Histologic evaluation of the BMs indicated adherence of M/MQs on BM surface, however no degradation was detected. Conclusion: For the first time, we have demonstrated that M/MQs are activated to varying levels by human BMs in vitro. These differences may be related to BM processing technologies and/or the biologic variation between donors. Our results raise the possibility that these differences in M/MQ activation could result in varying intensity of inflammation and wound healing that control the integration of BMs at sites of hernia repair.

Original languageEnglish (US)
Pages (from-to)10-14
Number of pages5
JournalJournal of Surgical Research
Volume158
Issue number1
DOIs
StatePublished - Jan 2010
Externally publishedYes

Fingerprint

Interleukin-1
Blood Cells
Herniorrhaphy
Wound Healing
Acellular Dermis
Tissue Donors
Inflammation
Monocytes
Healthy Volunteers
Enzyme-Linked Immunosorbent Assay
Macrophages
Cytokines
Technology
In Vitro Techniques
Alloderm
FlexHD

Keywords

  • biologic mesh
  • cytokine
  • dermal matrix
  • human
  • in vitro
  • Interleukin-1β
  • macrophage
  • monocyte
  • mononuclear cells

ASJC Scopus subject areas

  • Surgery

Cite this

In Vitro Activation of Human Peripheral Blood Mononuclear Cells Induced by Human Biologic Meshes1 . / Orenstein, Sean; Qiao, Yi; Kaur, Manjot; Klueh, Ulrike; Kreutzer, Don; Novitsky, Yuri.

In: Journal of Surgical Research, Vol. 158, No. 1, 01.2010, p. 10-14.

Research output: Contribution to journalArticle

Orenstein, Sean ; Qiao, Yi ; Kaur, Manjot ; Klueh, Ulrike ; Kreutzer, Don ; Novitsky, Yuri. / In Vitro Activation of Human Peripheral Blood Mononuclear Cells Induced by Human Biologic Meshes1 . In: Journal of Surgical Research. 2010 ; Vol. 158, No. 1. pp. 10-14.
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AU - Orenstein, Sean

AU - Qiao, Yi

AU - Kaur, Manjot

AU - Klueh, Ulrike

AU - Kreutzer, Don

AU - Novitsky, Yuri

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N2 - Background: Inflammation and wound healing play critical roles in the integration of biologic meshes (BMs) at sites of hernia repair. Monocytes/macrophages (M/MQs) are key cells involved in mesh integration. Interleukin-1β (IL-1β) is one of the major M/MQ-derived cytokines, and its expression is a reflection of the degree of M/MQ activation. We hypothesized that BMs induce M/MQ activation in vitro and that IL-1β expression by M/MQ varies among various BMs. Materials and Methods: Acellular human dermis-derived BM samples (AlloDerm, AlloMax, FlexHD) were placed in 48-well plates and cultured with peripheral blood mononuclear cells (PBMCs) from three healthy human subjects for 7 d. The resulting supernatants were assayed for IL-1β levels by enzyme-linked immunosorbent assay (ELISA), and the BMs were evaluated histologically. Results: IL-1β expression varied among donors as well as the BMs [AlloDerm (2.11-38.25pg/106 PBMCs); AlloMax (13.12-715.40pg/106 PBMCs); and FlexHD (116.69-665.40pg/106 PBMCs)]. Analysis of this data indicated that AlloMax and FlexHD induced significantly more M/MQ activation compared with AlloDerm (P <0.05). Histologic evaluation of the BMs indicated adherence of M/MQs on BM surface, however no degradation was detected. Conclusion: For the first time, we have demonstrated that M/MQs are activated to varying levels by human BMs in vitro. These differences may be related to BM processing technologies and/or the biologic variation between donors. Our results raise the possibility that these differences in M/MQ activation could result in varying intensity of inflammation and wound healing that control the integration of BMs at sites of hernia repair.

AB - Background: Inflammation and wound healing play critical roles in the integration of biologic meshes (BMs) at sites of hernia repair. Monocytes/macrophages (M/MQs) are key cells involved in mesh integration. Interleukin-1β (IL-1β) is one of the major M/MQ-derived cytokines, and its expression is a reflection of the degree of M/MQ activation. We hypothesized that BMs induce M/MQ activation in vitro and that IL-1β expression by M/MQ varies among various BMs. Materials and Methods: Acellular human dermis-derived BM samples (AlloDerm, AlloMax, FlexHD) were placed in 48-well plates and cultured with peripheral blood mononuclear cells (PBMCs) from three healthy human subjects for 7 d. The resulting supernatants were assayed for IL-1β levels by enzyme-linked immunosorbent assay (ELISA), and the BMs were evaluated histologically. Results: IL-1β expression varied among donors as well as the BMs [AlloDerm (2.11-38.25pg/106 PBMCs); AlloMax (13.12-715.40pg/106 PBMCs); and FlexHD (116.69-665.40pg/106 PBMCs)]. Analysis of this data indicated that AlloMax and FlexHD induced significantly more M/MQ activation compared with AlloDerm (P <0.05). Histologic evaluation of the BMs indicated adherence of M/MQs on BM surface, however no degradation was detected. Conclusion: For the first time, we have demonstrated that M/MQs are activated to varying levels by human BMs in vitro. These differences may be related to BM processing technologies and/or the biologic variation between donors. Our results raise the possibility that these differences in M/MQ activation could result in varying intensity of inflammation and wound healing that control the integration of BMs at sites of hernia repair.

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