In Situ Quantification of Genomic Instability in Breast Cancer Progression

C. Ortiz De Solórzano, K. Chin, J. W. Gray, S. J. Lockett

Research output: Contribution to journalConference articlepeer-review

Abstract

Genomic instability is a hallmark of breast and other solid cancers. Presumably caused by critical telomere reduction, GI is responsible for providing the genetic diversity required in the multi-step progression of the disease. We have used multicolor fluorescence in situ hybridization and 3D image analysis to quantify genomic instability cell-by-cell in thick, intact tissue sections of normal breast epithelium, preneoplastic lesions (usual ductal hyperplasia), ductal carcinona is situ or invasive carcinoma of the breast.. Our in situ -cell by cell- analysis of genomic instability shows an important increase of genomic instability in the transition from hyperplasia to in situ carcinoma, followed by a reduction of instability in invasive carcinoma. This pattern suggests that the transition from hyperplasia to in situ carcinoma corresponds to telomere crisis and invasive carcinoma is a consequence of telomerase reactivation afer telomere crisis.

Original languageEnglish (US)
Pages (from-to)3578-3581
Number of pages4
JournalAnnual International Conference of the IEEE Engineering in Medicine and Biology - Proceedings
Volume4
StatePublished - 2003
EventA New Beginning for Human Health: Proceedings of the 25th Annual International Conference of the IEEE Engineering in Medicine and Biology Society - Cancun, Mexico
Duration: Sep 17 2003Sep 21 2003

Keywords

  • 3D image segmentation
  • FISH
  • Genomic instability telomere crisis

ASJC Scopus subject areas

  • Signal Processing
  • Biomedical Engineering
  • Computer Vision and Pattern Recognition
  • Health Informatics

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