In Situ Quantification of Genomic Instability in Breast Cancer Progression

C. Ortiz De Solórzano, Kwang-Yung Chin, Joe Gray, S. J. Lockett

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Genomic instability is a hallmark of breast and other solid cancers. Presumably caused by critical telomere reduction, GI is responsible for providing the genetic diversity required in the multi-step progression of the disease. We have used multicolor fluorescence in situ hybridization and 3D image analysis to quantify genomic instability cell-by-cell in thick, intact tissue sections of normal breast epithelium, preneoplastic lesions (usual ductal hyperplasia), ductal carcinona is situ or invasive carcinoma of the breast.. Our in situ -cell by cell- analysis of genomic instability shows an important increase of genomic instability in the transition from hyperplasia to in situ carcinoma, followed by a reduction of instability in invasive carcinoma. This pattern suggests that the transition from hyperplasia to in situ carcinoma corresponds to telomere crisis and invasive carcinoma is a consequence of telomerase reactivation afer telomere crisis.

Original languageEnglish (US)
Title of host publicationAnnual International Conference of the IEEE Engineering in Medicine and Biology - Proceedings
EditorsR.S. Leder
Pages3578-3581
Number of pages4
Volume4
StatePublished - 2003
Externally publishedYes
EventA New Beginning for Human Health: Proceedings of the 25th Annual International Conference of the IEEE Engineering in Medicine and Biology Society - Cancun, Mexico
Duration: Sep 17 2003Sep 21 2003

Other

OtherA New Beginning for Human Health: Proceedings of the 25th Annual International Conference of the IEEE Engineering in Medicine and Biology Society
CountryMexico
CityCancun
Period9/17/039/21/03

Fingerprint

Telomerase
Image analysis
Fluorescence
Tissue

Keywords

  • 3D image segmentation
  • FISH
  • Genomic instability telomere crisis

ASJC Scopus subject areas

  • Bioengineering

Cite this

Ortiz De Solórzano, C., Chin, K-Y., Gray, J., & Lockett, S. J. (2003). In Situ Quantification of Genomic Instability in Breast Cancer Progression. In R. S. Leder (Ed.), Annual International Conference of the IEEE Engineering in Medicine and Biology - Proceedings (Vol. 4, pp. 3578-3581)

In Situ Quantification of Genomic Instability in Breast Cancer Progression. / Ortiz De Solórzano, C.; Chin, Kwang-Yung; Gray, Joe; Lockett, S. J.

Annual International Conference of the IEEE Engineering in Medicine and Biology - Proceedings. ed. / R.S. Leder. Vol. 4 2003. p. 3578-3581.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Ortiz De Solórzano, C, Chin, K-Y, Gray, J & Lockett, SJ 2003, In Situ Quantification of Genomic Instability in Breast Cancer Progression. in RS Leder (ed.), Annual International Conference of the IEEE Engineering in Medicine and Biology - Proceedings. vol. 4, pp. 3578-3581, A New Beginning for Human Health: Proceedings of the 25th Annual International Conference of the IEEE Engineering in Medicine and Biology Society, Cancun, Mexico, 9/17/03.
Ortiz De Solórzano C, Chin K-Y, Gray J, Lockett SJ. In Situ Quantification of Genomic Instability in Breast Cancer Progression. In Leder RS, editor, Annual International Conference of the IEEE Engineering in Medicine and Biology - Proceedings. Vol. 4. 2003. p. 3578-3581
Ortiz De Solórzano, C. ; Chin, Kwang-Yung ; Gray, Joe ; Lockett, S. J. / In Situ Quantification of Genomic Instability in Breast Cancer Progression. Annual International Conference of the IEEE Engineering in Medicine and Biology - Proceedings. editor / R.S. Leder. Vol. 4 2003. pp. 3578-3581
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