In situ monitoring of localized shear stress and fluid flow within developing tissue constructs by Doppler optical coherence tomography

Jia Yali, Pierre O. Bagnaninchi, Ruikang K. Wang

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Mechanical stimuli can be introduced to three dimensional (3D) cell cultures by use of perfusion bioreactor. Especially in musculoskeletal tissues, shear stress caused by fluid flow generally increase extra-cellular matrix (ECM) production and cell proliferation. The relationship between the shear stress and the tissue development in situ is complicated because of the non-uniform pore distribution within the cell-seeded scaffold. In this study, we firstly demonstrated that Doppler optical coherence tomography (DOCT) is capable of monitoring localized fluid flow and shear stress in the complex porous scaffold by examining their variation trends at perfusion rate of 5, 8, 10 and 12 ml/hr. Then, we developed the 3D porous cellular constructs, cell-seeded chitosan scaffolds monitored during several days by DOCT. The fiber based fourier domain DOCT employed a 1300 nm superluminescent diode with a bandwidth of 52 nm and a xyz resolution of 20×20×15 μm in free space. This setup allowed us not only to assess the cell growth and ECM deposition by observing their different scattering behaviors but also to further investigate how the cell attachment and ECM production has the effect on the flow shear stress and the relationship between flow rate and shear stress in the developing tissue construct. The possibility to monitor continuously the constructs under perfusion will easily indicate the effect of flow rate or shear stress on the cell viability and cell proliferation, and then discriminate the perfusion parameters affecting the pre-tissue formation rate growth.

Original languageEnglish (US)
Title of host publicationProgress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume6858
DOIs
StatePublished - 2008
EventOptics in Tissue Engineering and Regenerative Medicine II - San Jose, CA, United States
Duration: Jan 20 2008Jan 21 2008

Other

OtherOptics in Tissue Engineering and Regenerative Medicine II
CountryUnited States
CitySan Jose, CA
Period1/20/081/21/08

Fingerprint

Optical tomography
Shear stress
Flow of fluids
Tissue
Monitoring
Scaffolds
Cell proliferation
Flow rate
Cell growth
Bioreactors
Plastic flow
Chitosan
Cell culture
Diodes
Cells
Scattering
Bandwidth
Fibers

Keywords

  • Doppler optical coherence tomography
  • Local fluid flow
  • Porous scaffold
  • Shear stress
  • Tissue constructs

ASJC Scopus subject areas

  • Engineering(all)

Cite this

Yali, J., Bagnaninchi, P. O., & Wang, R. K. (2008). In situ monitoring of localized shear stress and fluid flow within developing tissue constructs by Doppler optical coherence tomography. In Progress in Biomedical Optics and Imaging - Proceedings of SPIE (Vol. 6858). [68580G] https://doi.org/10.1117/12.764720

In situ monitoring of localized shear stress and fluid flow within developing tissue constructs by Doppler optical coherence tomography. / Yali, Jia; Bagnaninchi, Pierre O.; Wang, Ruikang K.

Progress in Biomedical Optics and Imaging - Proceedings of SPIE. Vol. 6858 2008. 68580G.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Yali, J, Bagnaninchi, PO & Wang, RK 2008, In situ monitoring of localized shear stress and fluid flow within developing tissue constructs by Doppler optical coherence tomography. in Progress in Biomedical Optics and Imaging - Proceedings of SPIE. vol. 6858, 68580G, Optics in Tissue Engineering and Regenerative Medicine II, San Jose, CA, United States, 1/20/08. https://doi.org/10.1117/12.764720
Yali J, Bagnaninchi PO, Wang RK. In situ monitoring of localized shear stress and fluid flow within developing tissue constructs by Doppler optical coherence tomography. In Progress in Biomedical Optics and Imaging - Proceedings of SPIE. Vol. 6858. 2008. 68580G https://doi.org/10.1117/12.764720
Yali, Jia ; Bagnaninchi, Pierre O. ; Wang, Ruikang K. / In situ monitoring of localized shear stress and fluid flow within developing tissue constructs by Doppler optical coherence tomography. Progress in Biomedical Optics and Imaging - Proceedings of SPIE. Vol. 6858 2008.
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