In situ localization of actin mRNA in Dictyostelium discoideum aggregates

Eric Barklis, Harvey F. Lodish

Research output: Contribution to journalArticle

Abstract

By in situ hybridization of a labeled cloned DNA to cellular RNA, we have studied the distribution of actin mRNA in differentiating Dictyostelium discoideum aggregates. In migrating pseudoplasmodia, the mRNA is distributed uniformly. However, in culminating aggregates (sorogens) actin mRNA is enriched in stalk cells and in the cells at the anterior tip. Thus, the preferential synthesis of actin in prestalk and stalk cells, previously reported, is due at least partially to increased levels of actin mRNA.

Original languageEnglish (US)
Pages (from-to)479-486
Number of pages8
JournalExperimental Cell Research
Volume159
Issue number2
DOIs
StatePublished - Aug 1985

ASJC Scopus subject areas

  • Cell Biology

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