TY - JOUR
T1 - In situ localization of actin mRNA in Dictyostelium discoideum aggregates
AU - Barklis, Eric
AU - Lodish, Harvey F.
PY - 1985/8
Y1 - 1985/8
N2 - By in situ hybridization of a labeled cloned DNA to cellular RNA, we have studied the distribution of actin mRNA in differentiating Dictyostelium discoideum aggregates. In migrating pseudoplasmodia, the mRNA is distributed uniformly. However, in culminating aggregates (sorogens) actin mRNA is enriched in stalk cells and in the cells at the anterior tip. Thus, the preferential synthesis of actin in prestalk and stalk cells, previously reported, is due at least partially to increased levels of actin mRNA.
AB - By in situ hybridization of a labeled cloned DNA to cellular RNA, we have studied the distribution of actin mRNA in differentiating Dictyostelium discoideum aggregates. In migrating pseudoplasmodia, the mRNA is distributed uniformly. However, in culminating aggregates (sorogens) actin mRNA is enriched in stalk cells and in the cells at the anterior tip. Thus, the preferential synthesis of actin in prestalk and stalk cells, previously reported, is due at least partially to increased levels of actin mRNA.
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U2 - 10.1016/S0014-4827(85)80021-5
DO - 10.1016/S0014-4827(85)80021-5
M3 - Article
C2 - 4029275
AN - SCOPUS:0022005102
SN - 0014-4827
VL - 159
SP - 479
EP - 486
JO - Experimental Cell Research
JF - Experimental Cell Research
IS - 2
ER -