Improvement of antibody responses by HIV envelope DNA and protein co-immunization

Franco Pissani, Delphine Malherbe, Jason T. Schuman, Harlan Robins, Byung Park, Shelly J. Krebs, Susan W. Barnett, Nancy Haigwood

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

Background: Developing HIV envelope (Env) vaccine components that elicit durable and protective antibody responses is an urgent priority, given the results from the RV144 trial. Optimization of both the immunogens and vaccination strategies will be needed to generate potent, durable antibodies. Due to the diversity of HIV, an effective Env-based vaccine will most likely require an extensive coverage of antigenic variants. A vaccine co-delivering Env immunogens as DNA and protein components could provide such coverage. Here, we examine a DNA and protein co-immunization strategy by characterizing the antibody responses and evaluating the relative contribution of each vaccine component. Method: We co-immunized rabbits with representative subtype A or B HIV gp160 plasmid DNA plus Env gp140 trimeric glycoprotein and compared the responses to those obtained with either glycoprotein alone or glycoprotein in combination with empty vector. Results: DNA and glycoprotein co-immunization was superior to immunization with glycoprotein alone by enhancing antibody kinetics, magnitude, avidity, and neutralizing potency. Importantly, the empty DNA vector did not contribute to these responses. Humoral responses elicited by mismatched DNA and protein components were comparable or higher than the responses produced by the matched vaccines. Conclusion: Our data show that co-delivering DNA and protein can augment antibodies to Env. The rate and magnitude of immune responses suggest that this approach has the potential to streamline vaccine regimens by inducing higher antibody responses using fewer vaccinations, an advantage for a successful HIV vaccine design.

Original languageEnglish (US)
Pages (from-to)507-513
Number of pages7
JournalVaccine
Volume32
Issue number4
DOIs
StatePublished - Jan 16 2014

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Antibody Formation
Immunization
immunization
HIV
vaccines
glycoproteins
antibodies
Glycoproteins
Vaccines
DNA
Proteins
proteins
AIDS Vaccines
HIV Envelope Protein gp160
Vaccination
vaccination
antigens
Blocking Antibodies
Antibodies
humoral immunity

Keywords

  • DNA+protein co-immunization
  • Envelope-based vaccine
  • HIV
  • Neutralizing antibodies

ASJC Scopus subject areas

  • Immunology and Microbiology(all)
  • Infectious Diseases
  • Public Health, Environmental and Occupational Health
  • veterinary(all)
  • Molecular Medicine

Cite this

Improvement of antibody responses by HIV envelope DNA and protein co-immunization. / Pissani, Franco; Malherbe, Delphine; Schuman, Jason T.; Robins, Harlan; Park, Byung; Krebs, Shelly J.; Barnett, Susan W.; Haigwood, Nancy.

In: Vaccine, Vol. 32, No. 4, 16.01.2014, p. 507-513.

Research output: Contribution to journalArticle

Pissani, F, Malherbe, D, Schuman, JT, Robins, H, Park, B, Krebs, SJ, Barnett, SW & Haigwood, N 2014, 'Improvement of antibody responses by HIV envelope DNA and protein co-immunization', Vaccine, vol. 32, no. 4, pp. 507-513. https://doi.org/10.1016/j.vaccine.2013.11.022
Pissani, Franco ; Malherbe, Delphine ; Schuman, Jason T. ; Robins, Harlan ; Park, Byung ; Krebs, Shelly J. ; Barnett, Susan W. ; Haigwood, Nancy. / Improvement of antibody responses by HIV envelope DNA and protein co-immunization. In: Vaccine. 2014 ; Vol. 32, No. 4. pp. 507-513.
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