Improved resolution of flow cytometric measurements of hoechst‐ and chromomycin‐A3‐stained human chromosomes after addition of citrate and sulfite

Ger Van Den Engh, Barb Trask, Joe Gray, Peter Lansdorp

Research output: Contribution to journalArticle

48 Scopus citations

Abstract

The resolution of bivariate flow karyotypes of human chromosomes stained with Hoechst 33258 and chromomycin A3 can be increased by adding sodium citrate and sodium sulfite to the chromosomes shortly before measurement. A flow karyotype of a patient with chronic myelocytic leukemia is shown to illustrate that the addition of these compounds allows high‐resolution measurements to be made and evaluated reliably from clinical samples.

Original languageEnglish (US)
Pages (from-to)266-270
Number of pages5
JournalCytometry
Volume9
Issue number3
DOIs
StatePublished - May 1988

Keywords

  • Flow karyotype
  • chronic myeloid leukemia

ASJC Scopus subject areas

  • Pathology and Forensic Medicine
  • Biophysics
  • Hematology
  • Endocrinology
  • Cell Biology

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