Improved resolution of flow cytometric measurements of hoechst‐ and chromomycin‐A3‐stained human chromosomes after addition of citrate and sulfite

Ger Van Den Engh; Barb Trask; Joe Gray; Peter Lansdorp

doi:10.1002/cyto.990090313

Improved resolution of flow cytometric measurements of hoechst‐ and chromomycin‐A3‐stained human chromosomes after addition of citrate and sulfite

Ger Van Den Engh, Barb Trask, Joe Gray, Peter Lansdorp

Research output: Contribution to journal › Article › peer-review

55 Scopus citations

Abstract

The resolution of bivariate flow karyotypes of human chromosomes stained with Hoechst 33258 and chromomycin A3 can be increased by adding sodium citrate and sodium sulfite to the chromosomes shortly before measurement. A flow karyotype of a patient with chronic myelocytic leukemia is shown to illustrate that the addition of these compounds allows high‐resolution measurements to be made and evaluated reliably from clinical samples.

Original language	English (US)
Pages (from-to)	266-270
Number of pages	5
Journal	Cytometry
Volume	9
Issue number	3
DOIs	https://doi.org/10.1002/cyto.990090313
State	Published - May 1988
Externally published	Yes

Keywords

Flow karyotype
chronic myeloid leukemia

ASJC Scopus subject areas

Pathology and Forensic Medicine
Biophysics
Hematology
Endocrinology
Cell Biology

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10.1002/cyto.990090313

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abstract = "The resolution of bivariate flow karyotypes of human chromosomes stained with Hoechst 33258 and chromomycin A3 can be increased by adding sodium citrate and sodium sulfite to the chromosomes shortly before measurement. A flow karyotype of a patient with chronic myelocytic leukemia is shown to illustrate that the addition of these compounds allows high‐resolution measurements to be made and evaluated reliably from clinical samples.",

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AU - Engh, Ger Van Den

AU - Trask, Barb

AU - Gray, Joe

AU - Lansdorp, Peter

PY - 1988/5

Y1 - 1988/5

N2 - The resolution of bivariate flow karyotypes of human chromosomes stained with Hoechst 33258 and chromomycin A3 can be increased by adding sodium citrate and sodium sulfite to the chromosomes shortly before measurement. A flow karyotype of a patient with chronic myelocytic leukemia is shown to illustrate that the addition of these compounds allows high‐resolution measurements to be made and evaluated reliably from clinical samples.

AB - The resolution of bivariate flow karyotypes of human chromosomes stained with Hoechst 33258 and chromomycin A3 can be increased by adding sodium citrate and sodium sulfite to the chromosomes shortly before measurement. A flow karyotype of a patient with chronic myelocytic leukemia is shown to illustrate that the addition of these compounds allows high‐resolution measurements to be made and evaluated reliably from clinical samples.

KW - Flow karyotype

KW - chronic myeloid leukemia

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ER -

Improved resolution of flow cytometric measurements of hoechst‐ and chromomycin‐A3‐stained human chromosomes after addition of citrate and sulfite

Abstract

Keywords

ASJC Scopus subject areas

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