Improved murine glioma detection following modified diet and photobleaching of skin PpIX fluorescence

Summer Gibbs, Julia A. Ohara, P. Jack Hoopes, Brian W. Pogue

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

The Aminolevulinic Acid (ALA) - Protoporphyrin IX (PpIX) system is unique in the world of photosensitizers in that the prodrug ALA is enzymatically transformed via the tissue of interest into fluorescently detectable levels of PpIX. This system can be used to monitor cellular metabolism of tumor tissue for applications such as therapy monitoring. Detecting PpIX fluorescence noninvasively has proven difficult due to the high levels of PpIX produced in the skin compared to other tissue both with and without ALA administration. In the current study, methods to decrease skin PpIX autofluorescence and skin PpIX fluorescence following ALA administration have been examined. Use of a purified diet is found to decrease both skin PpIX autofluorescence and skin PpIX fluorescence following ALA administration, while addition of a broad spectrum antibiotic to the water shows little effect. Following ALA administration, improved brain tumor detection is seen when skin PpIX fluorescence is photobleached via blue light prior to transmission spectroscopic measurements of tumor bearing and control animals. Both of these methods to decrease skin PpIX autofluorescence and skin PpIX fluorescence following ALA administration are shown to have a large effect on the ability to detect tumor tissue PpIX fluorescence noninvasively in vivo.

Original languageEnglish (US)
Title of host publicationProgress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume6427
DOIs
StatePublished - 2007
Externally publishedYes
EventOptical Methods for Tumor Treatment and Detection: Mechanisms and Techniques in Photodynamic Therapy XVI - San Jose, CA, United States
Duration: Jan 20 2007Jan 21 2007

Other

OtherOptical Methods for Tumor Treatment and Detection: Mechanisms and Techniques in Photodynamic Therapy XVI
CountryUnited States
CitySan Jose, CA
Period1/20/071/21/07

Fingerprint

Photobleaching
Nutrition
Skin
Fluorescence
Acids
Tumors
Tissue
Bearings (structural)
Photosensitizers
Antibiotics
Metabolism
Brain
Animals
Monitoring

ASJC Scopus subject areas

  • Engineering(all)

Cite this

Gibbs, S., Ohara, J. A., Jack Hoopes, P., & Pogue, B. W. (2007). Improved murine glioma detection following modified diet and photobleaching of skin PpIX fluorescence. In Progress in Biomedical Optics and Imaging - Proceedings of SPIE (Vol. 6427). [64270D] https://doi.org/10.1117/12.700456

Improved murine glioma detection following modified diet and photobleaching of skin PpIX fluorescence. / Gibbs, Summer; Ohara, Julia A.; Jack Hoopes, P.; Pogue, Brian W.

Progress in Biomedical Optics and Imaging - Proceedings of SPIE. Vol. 6427 2007. 64270D.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Gibbs, S, Ohara, JA, Jack Hoopes, P & Pogue, BW 2007, Improved murine glioma detection following modified diet and photobleaching of skin PpIX fluorescence. in Progress in Biomedical Optics and Imaging - Proceedings of SPIE. vol. 6427, 64270D, Optical Methods for Tumor Treatment and Detection: Mechanisms and Techniques in Photodynamic Therapy XVI, San Jose, CA, United States, 1/20/07. https://doi.org/10.1117/12.700456
Gibbs S, Ohara JA, Jack Hoopes P, Pogue BW. Improved murine glioma detection following modified diet and photobleaching of skin PpIX fluorescence. In Progress in Biomedical Optics and Imaging - Proceedings of SPIE. Vol. 6427. 2007. 64270D https://doi.org/10.1117/12.700456
Gibbs, Summer ; Ohara, Julia A. ; Jack Hoopes, P. ; Pogue, Brian W. / Improved murine glioma detection following modified diet and photobleaching of skin PpIX fluorescence. Progress in Biomedical Optics and Imaging - Proceedings of SPIE. Vol. 6427 2007.
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