Improved murine glioma detection following modified diet and photobleaching of skin PpIX fluorescence

Summer L. Gibbs, Julia A. Ohara, P. Jack Hoopes, Brian W. Pogue

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

The Aminolevulinic Acid (ALA) - Protoporphyrin IX (PpIX) system is unique in the world of photosensitizers in that the prodrug ALA is enzymatically transformed via the tissue of interest into fluorescently detectable levels of PpIX. This system can be used to monitor cellular metabolism of tumor tissue for applications such as therapy monitoring. Detecting PpIX fluorescence noninvasively has proven difficult due to the high levels of PpIX produced in the skin compared to other tissue both with and without ALA administration. In the current study, methods to decrease skin PpIX autofluorescence and skin PpIX fluorescence following ALA administration have been examined. Use of a purified diet is found to decrease both skin PpIX autofluorescence and skin PpIX fluorescence following ALA administration, while addition of a broad spectrum antibiotic to the water shows little effect. Following ALA administration, improved brain tumor detection is seen when skin PpIX fluorescence is photobleached via blue light prior to transmission spectroscopic measurements of tumor bearing and control animals. Both of these methods to decrease skin PpIX autofluorescence and skin PpIX fluorescence following ALA administration are shown to have a large effect on the ability to detect tumor tissue PpIX fluorescence noninvasively in vivo.

Original languageEnglish (US)
Title of host publicationOptical Methods for Tumor Treatment and Detection
Subtitle of host publicationMechanisms and Techniques in Photodynamic Therapy XV
DOIs
StatePublished - Aug 31 2007
EventOptical Methods for Tumor Treatment and Detection: Mechanisms and Techniques in Photodynamic Therapy XVI - San Jose, CA, United States
Duration: Jan 20 2007Jan 21 2007

Publication series

NameProgress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume6427
ISSN (Print)1605-7422

Other

OtherOptical Methods for Tumor Treatment and Detection: Mechanisms and Techniques in Photodynamic Therapy XVI
CountryUnited States
CitySan Jose, CA
Period1/20/071/21/07

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ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Biomaterials
  • Atomic and Molecular Physics, and Optics
  • Radiology Nuclear Medicine and imaging

Cite this

Gibbs, S. L., Ohara, J. A., Jack Hoopes, P., & Pogue, B. W. (2007). Improved murine glioma detection following modified diet and photobleaching of skin PpIX fluorescence. In Optical Methods for Tumor Treatment and Detection: Mechanisms and Techniques in Photodynamic Therapy XV [64270D] (Progress in Biomedical Optics and Imaging - Proceedings of SPIE; Vol. 6427). https://doi.org/10.1117/12.700456