Abstract
A simple sperm/fertilization bioassay, primarily using sea urchin (and sand dollar) gametes, was improved to yield a quick, sensitive, and cost-effective procedure for measuring toxicity in marine waters. Standard sperm bioassays are conducted by exposing sperm cells to test solutions for 60 min prior to addition of eggs to the test solution for fertilization. Reduced fertilization success (as indicated by the presence or absence of the obvious fertilization membrane) is used as an indicator of toxic effects on sperm viability and/or the fertilization response. This study, in conjunction with earlier work, has shown that the results of sperm bioassays can be affected by a number of factors including temperature, pH, salinity, sperm:egg ratios, sperm exposure times, test materials, and echinoid species. Each of these factors have been considered in designing the "standard" conditions for the improved test. Examples of the effect of these factors on the test results are illustrated, using silver as a reference toxicant.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 23-32 |
| Number of pages | 10 |
| Journal | Archives of Environmental Contamination and Toxicology |
| Volume | 16 |
| Issue number | 1 |
| DOIs | |
| State | Published - Jan 1987 |
| Externally published | Yes |
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ASJC Scopus subject areas
- Toxicology
- Environmental Chemistry
- Environmental Science(all)
Cite this
Improved methodology for a sea urchin sperm cell bioassay for marine waters. / Dinnel, Paul A.; Link, Jeanne; Stober, Quentin J.
In: Archives of Environmental Contamination and Toxicology, Vol. 16, No. 1, 01.1987, p. 23-32.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Improved methodology for a sea urchin sperm cell bioassay for marine waters
AU - Dinnel, Paul A.
AU - Link, Jeanne
AU - Stober, Quentin J.
PY - 1987/1
Y1 - 1987/1
N2 - A simple sperm/fertilization bioassay, primarily using sea urchin (and sand dollar) gametes, was improved to yield a quick, sensitive, and cost-effective procedure for measuring toxicity in marine waters. Standard sperm bioassays are conducted by exposing sperm cells to test solutions for 60 min prior to addition of eggs to the test solution for fertilization. Reduced fertilization success (as indicated by the presence or absence of the obvious fertilization membrane) is used as an indicator of toxic effects on sperm viability and/or the fertilization response. This study, in conjunction with earlier work, has shown that the results of sperm bioassays can be affected by a number of factors including temperature, pH, salinity, sperm:egg ratios, sperm exposure times, test materials, and echinoid species. Each of these factors have been considered in designing the "standard" conditions for the improved test. Examples of the effect of these factors on the test results are illustrated, using silver as a reference toxicant.
AB - A simple sperm/fertilization bioassay, primarily using sea urchin (and sand dollar) gametes, was improved to yield a quick, sensitive, and cost-effective procedure for measuring toxicity in marine waters. Standard sperm bioassays are conducted by exposing sperm cells to test solutions for 60 min prior to addition of eggs to the test solution for fertilization. Reduced fertilization success (as indicated by the presence or absence of the obvious fertilization membrane) is used as an indicator of toxic effects on sperm viability and/or the fertilization response. This study, in conjunction with earlier work, has shown that the results of sperm bioassays can be affected by a number of factors including temperature, pH, salinity, sperm:egg ratios, sperm exposure times, test materials, and echinoid species. Each of these factors have been considered in designing the "standard" conditions for the improved test. Examples of the effect of these factors on the test results are illustrated, using silver as a reference toxicant.
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U2 - 10.1007/BF01055356
DO - 10.1007/BF01055356
M3 - Article
C2 - 3813656
AN - SCOPUS:0023205934
VL - 16
SP - 23
EP - 32
JO - Archives of Environmental Contamination and Toxicology
JF - Archives of Environmental Contamination and Toxicology
SN - 0090-4341
IS - 1
ER -