Improved lipid-mediated gene transfer into primary cultures of hippocampal neurons

Stefanie Kaech-Petrie, Jae Bum Kim, Michael Cariola, Evelyn Ralston

Research output: Contribution to journalArticle

41 Citations (Scopus)

Abstract

We have examined lipids as transfection agents to introduce recombinant plasmids into primary cultures of rat hippocampal neurons. By modifying the protocol for transfection mediated by the commercial reagent DOTAP, we were able to achieve a transfection efficiency of about 3%. Expression of various transfected gene products was sustained for several weeks in culture, the neurons developed normally and the transfected gene products were targeted to the appropriate subcellular compartment.

Original languageEnglish (US)
Pages (from-to)344-348
Number of pages5
JournalMolecular Brain Research
Volume35
Issue number1-2
DOIs
StatePublished - Jan 1996
Externally publishedYes

Fingerprint

Transfection
Lipids
Neurons
Genes
Plasmids
1,2-dioleoyloxy-3-(trimethylammonium)propane

Keywords

  • Beta-galactosidase
  • Hippocampus
  • Liposomes
  • Neuronal culture
  • Transfection

ASJC Scopus subject areas

  • Molecular Biology
  • Cellular and Molecular Neuroscience

Cite this

Improved lipid-mediated gene transfer into primary cultures of hippocampal neurons. / Kaech-Petrie, Stefanie; Kim, Jae Bum; Cariola, Michael; Ralston, Evelyn.

In: Molecular Brain Research, Vol. 35, No. 1-2, 01.1996, p. 344-348.

Research output: Contribution to journalArticle

Kaech-Petrie, Stefanie ; Kim, Jae Bum ; Cariola, Michael ; Ralston, Evelyn. / Improved lipid-mediated gene transfer into primary cultures of hippocampal neurons. In: Molecular Brain Research. 1996 ; Vol. 35, No. 1-2. pp. 344-348.
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