@article{21908f2b43154c9e9b53f59d7639ae87,
title = "Improved lipid-mediated gene transfer into primary cultures of hippocampal neurons",
abstract = "We have examined lipids as transfection agents to introduce recombinant plasmids into primary cultures of rat hippocampal neurons. By modifying the protocol for transfection mediated by the commercial reagent DOTAP, we were able to achieve a transfection efficiency of about 3%. Expression of various transfected gene products was sustained for several weeks in culture, the neurons developed normally and the transfected gene products were targeted to the appropriate subcellular compartment.",
keywords = "Beta-galactosidase, Hippocampus, Liposomes, Neuronal culture, Transfection",
author = "Stefanie Kaech and Kim, {Jae Bum} and Michael Cariola and Evelyn Ralston",
note = "Funding Information: We would like to thank Dr. T.S. Reese for continuous supporta nd interesitn this work and Dr. G.A. Banker and colleaguesfo r help in getting the culture system established. We thank Drs. L. MatsuuchiK, . Yamamotoa nd J. Horowitzf or the gift of plasmidsa nd Drs. D. Louvarda nd R. Davis for the gift of anti-ER and anti-transferrrine ceptor antibodiesW. e are gratefutl o Drs. A. Buonannoa nd D. Von Agostonf or helpful commentos n the manuscripatn d to Edwin David for help in the preparationo f the manuscriptS.. K. was supportedin, part, by fellowshipso f the Swiss National Science Foundationa nd the CIBA-Geigy-Jubil~iums-Stiftung.",
year = "1996",
month = jan,
doi = "10.1016/0169-328X(95)00238-N",
language = "English (US)",
volume = "35",
pages = "344--348",
journal = "Molecular Brain Research",
issn = "0169-328X",
publisher = "Elsevier",
number = "1-2",
}