Improved lipid-mediated gene transfer into primary cultures of hippocampal neurons

Stefanie Kaech; Jae Bum Kim; Michael Cariola; Evelyn Ralston

doi:10.1016/0169-328X(95)00238-N

Improved lipid-mediated gene transfer into primary cultures of hippocampal neurons

Stefanie Kaech, Jae Bum Kim, Michael Cariola, Evelyn Ralston

Research output: Contribution to journal › Article › peer-review

41 Scopus citations

Abstract

We have examined lipids as transfection agents to introduce recombinant plasmids into primary cultures of rat hippocampal neurons. By modifying the protocol for transfection mediated by the commercial reagent DOTAP, we were able to achieve a transfection efficiency of about 3%. Expression of various transfected gene products was sustained for several weeks in culture, the neurons developed normally and the transfected gene products were targeted to the appropriate subcellular compartment.

Original language	English (US)
Pages (from-to)	344-348
Number of pages	5
Journal	Molecular Brain Research
Volume	35
Issue number	1-2
DOIs	https://doi.org/10.1016/0169-328X(95)00238-N
State	Published - Jan 1996
Externally published	Yes

Keywords

Beta-galactosidase
Hippocampus
Liposomes
Neuronal culture
Transfection

ASJC Scopus subject areas

Molecular Biology
Cellular and Molecular Neuroscience

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10.1016/0169-328X(95)00238-N

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@article{21908f2b43154c9e9b53f59d7639ae87,

title = "Improved lipid-mediated gene transfer into primary cultures of hippocampal neurons",

abstract = "We have examined lipids as transfection agents to introduce recombinant plasmids into primary cultures of rat hippocampal neurons. By modifying the protocol for transfection mediated by the commercial reagent DOTAP, we were able to achieve a transfection efficiency of about 3%. Expression of various transfected gene products was sustained for several weeks in culture, the neurons developed normally and the transfected gene products were targeted to the appropriate subcellular compartment.",

keywords = "Beta-galactosidase, Hippocampus, Liposomes, Neuronal culture, Transfection",

author = "Stefanie Kaech and Kim, {Jae Bum} and Michael Cariola and Evelyn Ralston",

note = "Funding Information: We would like to thank Dr. T.S. Reese for continuous supporta nd interesitn this work and Dr. G.A. Banker and colleaguesfo r help in getting the culture system established. We thank Drs. L. MatsuuchiK, . Yamamotoa nd J. Horowitzf or the gift of plasmidsa nd Drs. D. Louvarda nd R. Davis for the gift of anti-ER and anti-transferrrine ceptor antibodiesW. e are gratefutl o Drs. A. Buonannoa nd D. Von Agostonf or helpful commentos n the manuscripatn d to Edwin David for help in the preparationo f the manuscriptS.. K. was supportedin, part, by fellowshipso f the Swiss National Science Foundationa nd the CIBA-Geigy-Jubil~iums-Stiftung.",

year = "1996",

month = jan,

doi = "10.1016/0169-328X(95)00238-N",

language = "English (US)",

volume = "35",

pages = "344--348",

journal = "Brain Research",

issn = "0006-8993",

publisher = "Elsevier",

number = "1-2",

}

TY - JOUR

T1 - Improved lipid-mediated gene transfer into primary cultures of hippocampal neurons

AU - Kaech, Stefanie

AU - Kim, Jae Bum

AU - Cariola, Michael

AU - Ralston, Evelyn

N1 - Funding Information: We would like to thank Dr. T.S. Reese for continuous supporta nd interesitn this work and Dr. G.A. Banker and colleaguesfo r help in getting the culture system established. We thank Drs. L. MatsuuchiK, . Yamamotoa nd J. Horowitzf or the gift of plasmidsa nd Drs. D. Louvarda nd R. Davis for the gift of anti-ER and anti-transferrrine ceptor antibodiesW. e are gratefutl o Drs. A. Buonannoa nd D. Von Agostonf or helpful commentos n the manuscripatn d to Edwin David for help in the preparationo f the manuscriptS.. K. was supportedin, part, by fellowshipso f the Swiss National Science Foundationa nd the CIBA-Geigy-Jubil~iums-Stiftung.

PY - 1996/1

Y1 - 1996/1

N2 - We have examined lipids as transfection agents to introduce recombinant plasmids into primary cultures of rat hippocampal neurons. By modifying the protocol for transfection mediated by the commercial reagent DOTAP, we were able to achieve a transfection efficiency of about 3%. Expression of various transfected gene products was sustained for several weeks in culture, the neurons developed normally and the transfected gene products were targeted to the appropriate subcellular compartment.

AB - We have examined lipids as transfection agents to introduce recombinant plasmids into primary cultures of rat hippocampal neurons. By modifying the protocol for transfection mediated by the commercial reagent DOTAP, we were able to achieve a transfection efficiency of about 3%. Expression of various transfected gene products was sustained for several weeks in culture, the neurons developed normally and the transfected gene products were targeted to the appropriate subcellular compartment.

KW - Beta-galactosidase

KW - Hippocampus

KW - Liposomes

KW - Neuronal culture

KW - Transfection

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U2 - 10.1016/0169-328X(95)00238-N

DO - 10.1016/0169-328X(95)00238-N

M3 - Article

C2 - 8717375

AN - SCOPUS:0030047422

SN - 0006-8993

VL - 35

SP - 344

EP - 348

JO - Brain Research

JF - Brain Research

IS - 1-2

ER -

Improved lipid-mediated gene transfer into primary cultures of hippocampal neurons

Abstract

Keywords

ASJC Scopus subject areas

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