The viral infectivity factor (Vif) of human immunodeficiency virus type 1 (HIV-1) neutralizes an unidentified antiviral pathway that occurs only in nonpermissive (NP) cells. Using a yeast two-hybrid screen of a human lymphocyte cDNA library, we identified several potential Vif partners. One, the nuclear body protein Sp140, was found specifically in all NP cells (n = 12 cell lines tested; P ≤ 0.001), and HIV-1 infection induced its partial dispersal from nuclear bodies into cytosolic colocalization with Vif. Our results implicate Spl40 in a response to HIV-1 that may be related to or coordinated with the pathway that inactivates HIV-1 lacking vif.
|Original language||English (US)|
|Number of pages||6|
|Journal||Journal of virology|
|State||Published - Nov 1 2002|
ASJC Scopus subject areas
- Insect Science