Implication of the lymphocyte-specific nuclear body protein Sp140 in an innate response to human immunodeficiency virus type 1

Navid Madani, Robert Millette, Emily J. Platt, Mariana Marin, Susan L. Kozak, Donald B. Bloch, David Kabat

Research output: Contribution to journalArticle

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Abstract

The viral infectivity factor (Vif) of human immunodeficiency virus type 1 (HIV-1) neutralizes an unidentified antiviral pathway that occurs only in nonpermissive (NP) cells. Using a yeast two-hybrid screen of a human lymphocyte cDNA library, we identified several potential Vif partners. One, the nuclear body protein Sp140, was found specifically in all NP cells (n = 12 cell lines tested; P ≤ 0.001), and HIV-1 infection induced its partial dispersal from nuclear bodies into cytosolic colocalization with Vif. Our results implicate Spl40 in a response to HIV-1 that may be related to or coordinated with the pathway that inactivates HIV-1 lacking vif.

Original languageEnglish (US)
Pages (from-to)11133-11138
Number of pages6
JournalJournal of Virology
Volume76
Issue number21
DOIs
StatePublished - Nov 2002

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body protein
Nuclear Proteins
Human immunodeficiency virus 1
HIV-1
lymphocytes
Lymphocytes
pathogenicity
Virus Diseases
Gene Library
cDNA libraries
Antiviral Agents
Yeasts
cell lines
cells
yeasts
Cell Line
infection

ASJC Scopus subject areas

  • Immunology

Cite this

Implication of the lymphocyte-specific nuclear body protein Sp140 in an innate response to human immunodeficiency virus type 1. / Madani, Navid; Millette, Robert; Platt, Emily J.; Marin, Mariana; Kozak, Susan L.; Bloch, Donald B.; Kabat, David.

In: Journal of Virology, Vol. 76, No. 21, 11.2002, p. 11133-11138.

Research output: Contribution to journalArticle

Madani, Navid ; Millette, Robert ; Platt, Emily J. ; Marin, Mariana ; Kozak, Susan L. ; Bloch, Donald B. ; Kabat, David. / Implication of the lymphocyte-specific nuclear body protein Sp140 in an innate response to human immunodeficiency virus type 1. In: Journal of Virology. 2002 ; Vol. 76, No. 21. pp. 11133-11138.
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