Impaired immunoproteasome assembly and immune responses in PA28(-/-) mice

Tobias Preckel, Wai Ping Fung-Leung, Zeling Cai, Antonella Vitiello, Luisa Salter-Cid, Ola Winqvist, Tom G. Wolfe, Matthias Von Herrath, Ana Angulo, Peter Ghazal, Jiing Dwan Lee, Anne M. Fourie, Ying Wu, Jesse Pang, Karen Ngo, Per A. Peterson, Klaus Frueh, Young Yang

Research output: Contribution to journalArticle

142 Citations (Scopus)

Abstract

in vitro PA28 binds and activates proteasomes. It is shown here that mice with a disrupted PA28b gene lack PA28a and PA28b polypeptides, demonstrating that PA28 functions as a hetero-oligomer in vivo. Processing of antigenic epitopes derived from exogenous or endogenous antigens is altered in PA28(-/-) mice. Cytotoxic T lymphocyte responses are impaired, and assembly of immunoproteasomes is greatly inhibited in mice lacking PA28. These results show that PA28 is necessary for immunoproteasome assembly and is required for efficient antigen processing, thus demonstrating the importance of PA28-mediated proteasome function in immune responses.

Original languageEnglish (US)
Pages (from-to)2162-2165
Number of pages4
JournalScience
Volume286
Issue number5447
DOIs
StatePublished - Dec 10 1999
Externally publishedYes

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Proteasome Endopeptidase Complex
Antigen Presentation
Cytotoxic T-Lymphocytes
Epitopes
Antigens
Peptides
Genes
In Vitro Techniques

ASJC Scopus subject areas

  • General

Cite this

Preckel, T., Fung-Leung, W. P., Cai, Z., Vitiello, A., Salter-Cid, L., Winqvist, O., ... Yang, Y. (1999). Impaired immunoproteasome assembly and immune responses in PA28(-/-) mice. Science, 286(5447), 2162-2165. https://doi.org/10.1126/science.286.5447.2162

Impaired immunoproteasome assembly and immune responses in PA28(-/-) mice. / Preckel, Tobias; Fung-Leung, Wai Ping; Cai, Zeling; Vitiello, Antonella; Salter-Cid, Luisa; Winqvist, Ola; Wolfe, Tom G.; Von Herrath, Matthias; Angulo, Ana; Ghazal, Peter; Lee, Jiing Dwan; Fourie, Anne M.; Wu, Ying; Pang, Jesse; Ngo, Karen; Peterson, Per A.; Frueh, Klaus; Yang, Young.

In: Science, Vol. 286, No. 5447, 10.12.1999, p. 2162-2165.

Research output: Contribution to journalArticle

Preckel, T, Fung-Leung, WP, Cai, Z, Vitiello, A, Salter-Cid, L, Winqvist, O, Wolfe, TG, Von Herrath, M, Angulo, A, Ghazal, P, Lee, JD, Fourie, AM, Wu, Y, Pang, J, Ngo, K, Peterson, PA, Frueh, K & Yang, Y 1999, 'Impaired immunoproteasome assembly and immune responses in PA28(-/-) mice', Science, vol. 286, no. 5447, pp. 2162-2165. https://doi.org/10.1126/science.286.5447.2162
Preckel T, Fung-Leung WP, Cai Z, Vitiello A, Salter-Cid L, Winqvist O et al. Impaired immunoproteasome assembly and immune responses in PA28(-/-) mice. Science. 1999 Dec 10;286(5447):2162-2165. https://doi.org/10.1126/science.286.5447.2162
Preckel, Tobias ; Fung-Leung, Wai Ping ; Cai, Zeling ; Vitiello, Antonella ; Salter-Cid, Luisa ; Winqvist, Ola ; Wolfe, Tom G. ; Von Herrath, Matthias ; Angulo, Ana ; Ghazal, Peter ; Lee, Jiing Dwan ; Fourie, Anne M. ; Wu, Ying ; Pang, Jesse ; Ngo, Karen ; Peterson, Per A. ; Frueh, Klaus ; Yang, Young. / Impaired immunoproteasome assembly and immune responses in PA28(-/-) mice. In: Science. 1999 ; Vol. 286, No. 5447. pp. 2162-2165.
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