TY - JOUR
T1 - Immunosuppression by canine distemper virus
T2 - Modulation of in vitro immunoglobulin synthesis, interleukin release and prostaglandin E2 production
AU - Krakowka, Steven
AU - Ringler, Susan S.
AU - Lewis, Mark
AU - Olsen, Richard G.
AU - Axthelm, Michael K.
N1 - Funding Information:
ACKN~S The State of Ohio Canine Research Fund and Grant No. 2RO1-14821, NIH, PHS supported this research. The authors wish to thank Mr. Richard Bauer for performance of the CTLL assay for IL-2 and Ms. Nancy J. Austin and Judith R. Dubena for excellent technical assistance.
PY - 1987/6
Y1 - 1987/6
N2 - In vitro or in vivo infection of canine mononuclear cells by canine distemper virus (CDV) in short-term microcultures resulted in suppression of lectin-induced 3H-thymidine incorporation. This suppressive effect was also evident in pokeweed mitogen-driven in vitro immunoglobulin synthesis and release. Lectin-induced interleukin-2 production by monocyte-depleted lymphocyte cultures was marginally affected by CDV, whereas interleukin-1 production by adherent mononuclear cells was significantly depressed. Monocyte cultures established from viremic dogs released prostaglandin (PG)E2. The results suggest that, in addition to a direct viral effect upon lectin responsive cellular population(s), CDV modulates monocyte functions by inhibition of interleukin-1 production and by enhancing PGE2 release.
AB - In vitro or in vivo infection of canine mononuclear cells by canine distemper virus (CDV) in short-term microcultures resulted in suppression of lectin-induced 3H-thymidine incorporation. This suppressive effect was also evident in pokeweed mitogen-driven in vitro immunoglobulin synthesis and release. Lectin-induced interleukin-2 production by monocyte-depleted lymphocyte cultures was marginally affected by CDV, whereas interleukin-1 production by adherent mononuclear cells was significantly depressed. Monocyte cultures established from viremic dogs released prostaglandin (PG)E2. The results suggest that, in addition to a direct viral effect upon lectin responsive cellular population(s), CDV modulates monocyte functions by inhibition of interleukin-1 production and by enhancing PGE2 release.
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U2 - 10.1016/0165-2427(87)90082-1
DO - 10.1016/0165-2427(87)90082-1
M3 - Article
C2 - 3498254
AN - SCOPUS:0023272391
SN - 0165-2427
VL - 15
SP - 181
EP - 201
JO - Veterinary Immunology and Immunopathology
JF - Veterinary Immunology and Immunopathology
IS - 3
ER -