Immunopathology and electron microscopy of Acanthamoeba keratitis

W. Mathers, G. Stevens, M. Rodrigues, C. C. Chan, J. Gold, G. S. Visvesvara, M. A. Lemp, L. E. Zimmerman

Research output: Contribution to journalArticlepeer-review

63 Scopus citations


In order to evaluate the local cellular immune response to Acanthamoeba infection we performed immunohistochemical examinations of the corneal buttons of two patients with Acanthamoeba keratitis. We found that the corneal stroma was infiltrated with polymorphonuclear leukocytes and HLA-DR positive macrophages that appeared to be stromal keratocytes by light microscopy. Despite the presence of chronic inflammation in both patients, no stromal lymphocytes were seen in one patient and a sparse lymphocytic infiltrate was seen in the other patient. Electron microscopy confirmed the presence of macrophages, neutrophils, and Acanthamoeba organisms in these two patients.

Original languageEnglish (US)
Pages (from-to)626-635
Number of pages10
JournalAmerican journal of ophthalmology
Issue number5 III
StatePublished - 1987
Externally publishedYes

ASJC Scopus subject areas

  • Ophthalmology


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