Immunological studies with a monoclonal antibody suggest a different conformation of neurophysin in parvicellular neurons of rat hypothalamus

Gajanan Nilaver, Lawrence C. Rosenbaum, Hubert H.M. Van Tol, Eva Marie Shannon, Heidi M. Hagman, Earl A. Zimmerman, Edward A. Neuwelt

Research output: Contribution to journalArticle

Abstract

A monoclonal antibody (mAb L6) to a carcinoma surface antigen has previously been shown to recognize neurophysins (NP), proteins associated with oxytocin and vasopressin14. L6-reactivity in rat hypothalamus was confined to magnocellular neuronal systems. No staining was detected in parvicellular syprachiasmatic or paraventricular systems. mAb L6 immunoprecipitated vasopressin-neurophysin only under reducing conditions, and detected it in Western blots only after gel-renaturation and electroblotting in basic buffer. These findings suggest L6-reactivity to NP is conformation-sensitive, and imply NP expression in a unique configurational form in hypothalamic parvicellular systems.

Original languageEnglish (US)
Pages (from-to)302-308
Number of pages7
JournalBrain research
Volume529
Issue number1-2
DOIs
StatePublished - Oct 8 1990

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Keywords

  • Hypothalamus
  • Immunohistochemistry
  • Immunoprecipitation
  • Monoclonal antibody
  • Neurophysin
  • Paraventricular nucleus
  • Suprachiasmatic nucleus
  • Supraoptic nucleus
  • Western blot

ASJC Scopus subject areas

  • Neuroscience(all)
  • Molecular Biology
  • Clinical Neurology
  • Developmental Biology

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