Immunologic memory to phosphorylcholine. VI. Heterogeneity in light chain gene expression

I. Todd, M. Brown, Marvin Rittenberg

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

BALB/c mice immunized with phosphorylcholine-conjugated keyhole limpet hemocyanin (PC-KLH) produce two types of anti-PC antibodies, designated group I and group II, which differ in their fine specificity and idiotype expression. Group II hybridomas can utilize V(H) genes and V(L) genes (in particular, V(κ)1-3) distinct from those expressed in the group I-like anti-PC myelomas. Here we have analyzed additional anti-PC hybridomas from BALB/c and (CBAN x BALB/c)F1 male mice and also anti-PC antibodies purified from BALB/c and C57BL/6N antisera. Isoelectric focusing indicates that one of the group II hybridomas utilizes V(κ)1-3 and that related L chains are expressed in a major portion of group II serum antibodies. Other group II antibodies in antisera express different L chains, some of which are presently unidentified. However, isoelectric focusing analysis also indicates that the L chains of some group II hybridomas and group I hybridoma and serum antibodies. In addition, one hybridoma was found to utilize λ2. Thus, it appears that the anti-PC antibodies with group II-like fine specificity can utilize a variety of V(L) genes related to, or distinct from, those expressed in group I antibodies.

Original languageEnglish (US)
Pages (from-to)177-183
Number of pages7
JournalEuropean Journal of Immunology
Volume15
Issue number2
StatePublished - 1985

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Immunologic Memory
Phosphorylcholine
Hybridomas
Gene Expression
Light
Anti-Idiotypic Antibodies
Antibodies
Isoelectric Focusing
Immune Sera
Genes
Serum

ASJC Scopus subject areas

  • Immunology

Cite this

Immunologic memory to phosphorylcholine. VI. Heterogeneity in light chain gene expression. / Todd, I.; Brown, M.; Rittenberg, Marvin.

In: European Journal of Immunology, Vol. 15, No. 2, 1985, p. 177-183.

Research output: Contribution to journalArticle

Todd, I. ; Brown, M. ; Rittenberg, Marvin. / Immunologic memory to phosphorylcholine. VI. Heterogeneity in light chain gene expression. In: European Journal of Immunology. 1985 ; Vol. 15, No. 2. pp. 177-183.
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