Immunologic memory to phosphorylcholine. VI. Heterogeneity in light chain gene expression

Ian Todd, McKay Brown, Marvin B. Rittenberg

Research output: Contribution to journalArticle

9 Scopus citations

Abstract

BALB/c mice immunized with phosphorylcholine‐conjugated keyhole limpet hemocy‐anin (PC‐KLH) produce two types of anti‐PC antibodies, designated group I and group II, which differ in their fine specificity and idiotype expression. Group I1 hybridomas can utilize VH genes and VL genes (in particular, Vϰ1–3) distinct from those expressed in the group I‐like anti‐PC myelomas. Here we have analyzed additional anti‐PC hybridomas from BALB/c and (CBA/N × BALB/c)F1 male mice and also anti‐PC antibodies purified from BALB/c and C57BL/6N antisera. Isoelectric focusing indicates that one of the group II hybridomas utilizes Vϰ1–3 and that related L chains are expressed in a major portion of group II serum antibodies. Other group I1 antibodies in antisera express different L chains, some of which are presently unidentified. However, isoelectric focusing analysis also indicates that the L chains of some group I1 hybridomas and serum antibodies are related to those found in the group I anti‐PC myelomas and group I hybridoma and serum antibodies. In addition, one hybridoma was found to utilize λ2. Thus, it appears that the anti‐PC antibodies with group II‐like fine specificity can utilize a variety of VL genes related to, or distinct from, those expressed in group I antibodies.

Original languageEnglish (US)
Pages (from-to)177-183
Number of pages7
JournalEuropean Journal of Immunology
Volume15
Issue number2
DOIs
StatePublished - 1985

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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