Immunologic memory to phosphocholine. V. Hybridomas representative of group II antibodies utilize V(k)1-3 gene(s)

I. Todd, S. P. Chang, R. M. Perlmutter, R. Aebersold, C. H. Heusser, L. Hood, Marvin Rittenberg

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

The anti-phosphocholine (PC) memory response of BALB/c mice to PC-KLH contains two groups of antibodies distinguished by fine specificity and by expression of the T15 idiotype that dominates Group I but not Group II anti-PC antibodies. The contribution of V(k) genes to this diversity was investigated by the analysis of L chains from PC-binding hybridoma proteins (PCBHP) representative of Group I and Group II. N-terminal amino acid sequence analysis was performed on the L chains of three independently derived Group II PCBHP up to residue 23 (PCG1-1) or 21 (aPC-111-1 and aPC-12-3). These three sequences differed from each other by only one or two residues, but differed by approximately 50% from the L chains of the Group I-like PC-binding myeloma proteins (PCBMP); the Group II sequences are closely related to V(k)1-3. Isoelectric focusing analysis was also performed on the L chain of PCG1-1, as well as on L chains from PCBHP typical of Group I antibodies, and from an atypical PCBHP differing from Groups I and II in fine specificity. A Group I PCBHP and the atypical PCBHP expressed L chains related to V(k)8 and V(k)24, respectively. The L chains of another Group I PCBHP and of the Group II protein, PCG1-1, appeared different from those found in the PCBMP and from each other. The results indicate a more diverse expression of L chains in the memory anti-PC response than is represented by the PCBMP; both V(k)8- and V(k)24-derived L chains (and, presumably, somatic variants), as well as products of additional V(k) genes (V(k)1-3), appear to be present in the anti-PC memory pool.

Original languageEnglish (US)
Pages (from-to)1556-1560
Number of pages5
JournalJournal of Immunology
Volume132
Issue number3
StatePublished - 1984
Externally publishedYes

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Immunologic Memory
Phosphorylcholine
Hybridomas
Carrier Proteins
Antibodies
Genes
Myeloma Proteins
Protein Sequence Analysis
Isoelectric Focusing
Anti-Idiotypic Antibodies

ASJC Scopus subject areas

  • Immunology

Cite this

Todd, I., Chang, S. P., Perlmutter, R. M., Aebersold, R., Heusser, C. H., Hood, L., & Rittenberg, M. (1984). Immunologic memory to phosphocholine. V. Hybridomas representative of group II antibodies utilize V(k)1-3 gene(s). Journal of Immunology, 132(3), 1556-1560.

Immunologic memory to phosphocholine. V. Hybridomas representative of group II antibodies utilize V(k)1-3 gene(s). / Todd, I.; Chang, S. P.; Perlmutter, R. M.; Aebersold, R.; Heusser, C. H.; Hood, L.; Rittenberg, Marvin.

In: Journal of Immunology, Vol. 132, No. 3, 1984, p. 1556-1560.

Research output: Contribution to journalArticle

Todd, I, Chang, SP, Perlmutter, RM, Aebersold, R, Heusser, CH, Hood, L & Rittenberg, M 1984, 'Immunologic memory to phosphocholine. V. Hybridomas representative of group II antibodies utilize V(k)1-3 gene(s)', Journal of Immunology, vol. 132, no. 3, pp. 1556-1560.
Todd I, Chang SP, Perlmutter RM, Aebersold R, Heusser CH, Hood L et al. Immunologic memory to phosphocholine. V. Hybridomas representative of group II antibodies utilize V(k)1-3 gene(s). Journal of Immunology. 1984;132(3):1556-1560.
Todd, I. ; Chang, S. P. ; Perlmutter, R. M. ; Aebersold, R. ; Heusser, C. H. ; Hood, L. ; Rittenberg, Marvin. / Immunologic memory to phosphocholine. V. Hybridomas representative of group II antibodies utilize V(k)1-3 gene(s). In: Journal of Immunology. 1984 ; Vol. 132, No. 3. pp. 1556-1560.
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abstract = "The anti-phosphocholine (PC) memory response of BALB/c mice to PC-KLH contains two groups of antibodies distinguished by fine specificity and by expression of the T15 idiotype that dominates Group I but not Group II anti-PC antibodies. The contribution of V(k) genes to this diversity was investigated by the analysis of L chains from PC-binding hybridoma proteins (PCBHP) representative of Group I and Group II. N-terminal amino acid sequence analysis was performed on the L chains of three independently derived Group II PCBHP up to residue 23 (PCG1-1) or 21 (aPC-111-1 and aPC-12-3). These three sequences differed from each other by only one or two residues, but differed by approximately 50{\%} from the L chains of the Group I-like PC-binding myeloma proteins (PCBMP); the Group II sequences are closely related to V(k)1-3. Isoelectric focusing analysis was also performed on the L chain of PCG1-1, as well as on L chains from PCBHP typical of Group I antibodies, and from an atypical PCBHP differing from Groups I and II in fine specificity. A Group I PCBHP and the atypical PCBHP expressed L chains related to V(k)8 and V(k)24, respectively. The L chains of another Group I PCBHP and of the Group II protein, PCG1-1, appeared different from those found in the PCBMP and from each other. The results indicate a more diverse expression of L chains in the memory anti-PC response than is represented by the PCBMP; both V(k)8- and V(k)24-derived L chains (and, presumably, somatic variants), as well as products of additional V(k) genes (V(k)1-3), appear to be present in the anti-PC memory pool.",
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AU - Heusser, C. H.

AU - Hood, L.

AU - Rittenberg, Marvin

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