Immunolocalization of transforming growth factor-α and its receptor in the normal and hyperoxia-exposed neonatal rat retina

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Abstract

Purpose. Transforming growth factor-α (TGF-α) is a mitogenic polypeptide for a variety of different cells types including retinal neurons and glial cells. We have examined the temporal and spatial expression of TGF-α and its receptor in the normal and hyperoxia-exposed neonatal rat retina to determine if the expression is consistent with a role in retinal development and response to retinal injury. Methods. We have used immunohistochemistry to examine TGF-α and epidermal growth factor receptor (EGF-R) on postnatal days (1, 5, 10, 14, 18, and 25). To examine TGF-α and EGF-R expression after retinal injury we studied the retinas from rats which were exposed to 80% oxygen for 10 days and then recovered in room air. Immunolocalization of type IV collagen was performed to examine the retinal vasculature development after hyperoxia. Results. The pattern of TGF-α and EGF-R expression in the neural retina evolved from a diffuse pattern on postnatal day 1 to restricted sites on postnatal day 14. The TGF-α immunoreactivity was consistent with localization in Muller cells on postnatal day 14. Both TGF-α and EGF-R patterns were altered in the retinas from rats that had been exposed to hyperoxia and recovered in room air for 4 days. The type IV confirmed immunostaining confirmed vase-obliteration in the deep layer of retinal vessels after hyperoxia. Conclusions. Our findings of altered expression of TGF-α and EGF-R during retinal development suggests a biological function for this growth factor, possibly promoting retinal cell proliferation, differentiation, and survival. The altered immunolocalization of TGF-α and EGF-R in the hyperoxia-exposed retina suggest that TGF-α is likely involved in the retinal response to injury.

Original languageEnglish (US)
Pages (from-to)177-182
Number of pages6
JournalCurrent Eye Research
Volume16
Issue number3
DOIs
StatePublished - 1997

Fingerprint

Hyperoxia
Growth Factor Receptors
Transforming Growth Factors
Retina
Epidermal Growth Factor Receptor
Wounds and Injuries
Air
Ependymoglial Cells
Retinal Neurons
Retinal Vessels
Collagen Type IV
Neuroglia
Cell Differentiation
Cell Survival
Intercellular Signaling Peptides and Proteins
Immunohistochemistry
Cell Proliferation
Oxygen
Peptides

Keywords

  • Epidermal growth factor-receptor
  • Hyperoxia
  • Rat
  • Retina
  • Retinal development
  • Transforming growth factor-α

ASJC Scopus subject areas

  • Ophthalmology
  • Sensory Systems

Cite this

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title = "Immunolocalization of transforming growth factor-α and its receptor in the normal and hyperoxia-exposed neonatal rat retina",
abstract = "Purpose. Transforming growth factor-α (TGF-α) is a mitogenic polypeptide for a variety of different cells types including retinal neurons and glial cells. We have examined the temporal and spatial expression of TGF-α and its receptor in the normal and hyperoxia-exposed neonatal rat retina to determine if the expression is consistent with a role in retinal development and response to retinal injury. Methods. We have used immunohistochemistry to examine TGF-α and epidermal growth factor receptor (EGF-R) on postnatal days (1, 5, 10, 14, 18, and 25). To examine TGF-α and EGF-R expression after retinal injury we studied the retinas from rats which were exposed to 80{\%} oxygen for 10 days and then recovered in room air. Immunolocalization of type IV collagen was performed to examine the retinal vasculature development after hyperoxia. Results. The pattern of TGF-α and EGF-R expression in the neural retina evolved from a diffuse pattern on postnatal day 1 to restricted sites on postnatal day 14. The TGF-α immunoreactivity was consistent with localization in Muller cells on postnatal day 14. Both TGF-α and EGF-R patterns were altered in the retinas from rats that had been exposed to hyperoxia and recovered in room air for 4 days. The type IV confirmed immunostaining confirmed vase-obliteration in the deep layer of retinal vessels after hyperoxia. Conclusions. Our findings of altered expression of TGF-α and EGF-R during retinal development suggests a biological function for this growth factor, possibly promoting retinal cell proliferation, differentiation, and survival. The altered immunolocalization of TGF-α and EGF-R in the hyperoxia-exposed retina suggest that TGF-α is likely involved in the retinal response to injury.",
keywords = "Epidermal growth factor-receptor, Hyperoxia, Rat, Retina, Retinal development, Transforming growth factor-α",
author = "Powers, {Michael (Mike)} and Stephen Planck",
year = "1997",
doi = "10.1076/ceyr.16.3.177.15406",
language = "English (US)",
volume = "16",
pages = "177--182",
journal = "Current Eye Research",
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T1 - Immunolocalization of transforming growth factor-α and its receptor in the normal and hyperoxia-exposed neonatal rat retina

AU - Powers, Michael (Mike)

AU - Planck, Stephen

PY - 1997

Y1 - 1997

N2 - Purpose. Transforming growth factor-α (TGF-α) is a mitogenic polypeptide for a variety of different cells types including retinal neurons and glial cells. We have examined the temporal and spatial expression of TGF-α and its receptor in the normal and hyperoxia-exposed neonatal rat retina to determine if the expression is consistent with a role in retinal development and response to retinal injury. Methods. We have used immunohistochemistry to examine TGF-α and epidermal growth factor receptor (EGF-R) on postnatal days (1, 5, 10, 14, 18, and 25). To examine TGF-α and EGF-R expression after retinal injury we studied the retinas from rats which were exposed to 80% oxygen for 10 days and then recovered in room air. Immunolocalization of type IV collagen was performed to examine the retinal vasculature development after hyperoxia. Results. The pattern of TGF-α and EGF-R expression in the neural retina evolved from a diffuse pattern on postnatal day 1 to restricted sites on postnatal day 14. The TGF-α immunoreactivity was consistent with localization in Muller cells on postnatal day 14. Both TGF-α and EGF-R patterns were altered in the retinas from rats that had been exposed to hyperoxia and recovered in room air for 4 days. The type IV confirmed immunostaining confirmed vase-obliteration in the deep layer of retinal vessels after hyperoxia. Conclusions. Our findings of altered expression of TGF-α and EGF-R during retinal development suggests a biological function for this growth factor, possibly promoting retinal cell proliferation, differentiation, and survival. The altered immunolocalization of TGF-α and EGF-R in the hyperoxia-exposed retina suggest that TGF-α is likely involved in the retinal response to injury.

AB - Purpose. Transforming growth factor-α (TGF-α) is a mitogenic polypeptide for a variety of different cells types including retinal neurons and glial cells. We have examined the temporal and spatial expression of TGF-α and its receptor in the normal and hyperoxia-exposed neonatal rat retina to determine if the expression is consistent with a role in retinal development and response to retinal injury. Methods. We have used immunohistochemistry to examine TGF-α and epidermal growth factor receptor (EGF-R) on postnatal days (1, 5, 10, 14, 18, and 25). To examine TGF-α and EGF-R expression after retinal injury we studied the retinas from rats which were exposed to 80% oxygen for 10 days and then recovered in room air. Immunolocalization of type IV collagen was performed to examine the retinal vasculature development after hyperoxia. Results. The pattern of TGF-α and EGF-R expression in the neural retina evolved from a diffuse pattern on postnatal day 1 to restricted sites on postnatal day 14. The TGF-α immunoreactivity was consistent with localization in Muller cells on postnatal day 14. Both TGF-α and EGF-R patterns were altered in the retinas from rats that had been exposed to hyperoxia and recovered in room air for 4 days. The type IV confirmed immunostaining confirmed vase-obliteration in the deep layer of retinal vessels after hyperoxia. Conclusions. Our findings of altered expression of TGF-α and EGF-R during retinal development suggests a biological function for this growth factor, possibly promoting retinal cell proliferation, differentiation, and survival. The altered immunolocalization of TGF-α and EGF-R in the hyperoxia-exposed retina suggest that TGF-α is likely involved in the retinal response to injury.

KW - Epidermal growth factor-receptor

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