Immunolocalization of natriuretic peptide receptor B in the rat kidney

Marie Chantal Bonhomme, Kevin Grove, Sylvie Caron, Caroline T. Crilley, Gaetan Thibault, Christian F. Deschepper, Raul Garcia

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

The natriuretic peptide receptor (NPR) family consists of three receptor subtypes: two transmembrane forms that contain a guanylyl cyclase intracellular domain (NPR-A and NPR-B), and one truncated form (NPR-C). Because of the lack of specific agonists and antagonists for each receptor subtype and to the difficulty to detect the presence of small quantities of NPR-B by ligand binding studies, polyclonal antibodies against a peptide whose sequence was chosen from a region of the extracellular domain of rat NPR-B that is not homologous to sequences in NPR-A and NPR-C were developed Western blotting with affinity-purified anti-NPR-B (413-426)-Tyr revealed a polypeptide of approximately 120 kD on COS-1 cell membranes transfected with rat NPR-B cDNA. The antibody recognized a second polypeptide, approximately 5 to 10 kD smaller, which probably represents the unglycosylated receptor. Anti-NPR-B (413-426)-Tyr did not show crossreactivity to any other NPR. Western blotting analysis with anti-NPR-B (413-426)-Tyr also identified a protein of appropriate size in renal vascular membranes. These results were supported by immunohistochemistry findings that demonstrated staining for NPR-B on papillary and medullary capillaries, glomeruli, and renal arteries. This study concludes that NPR-B is present in the rat kidney, although it was only detected in vascular structures.

Original languageEnglish (US)
Pages (from-to)1777-1786
Number of pages10
JournalJournal of the American Society of Nephrology
Volume9
Issue number10
StatePublished - Oct 1998
Externally publishedYes

Fingerprint

Kidney
Natriuretic Peptides
Peptide Receptors
Peptides
Blood Vessels
Western Blotting
atrial natriuretic factor receptor B
Antibodies
Guanylate Cyclase
COS Cells
Renal Artery
Sequence Homology
Complementary DNA
Immunohistochemistry
Cell Membrane
Staining and Labeling
Ligands
Membranes
Proteins

ASJC Scopus subject areas

  • Nephrology

Cite this

Bonhomme, M. C., Grove, K., Caron, S., Crilley, C. T., Thibault, G., Deschepper, C. F., & Garcia, R. (1998). Immunolocalization of natriuretic peptide receptor B in the rat kidney. Journal of the American Society of Nephrology, 9(10), 1777-1786.

Immunolocalization of natriuretic peptide receptor B in the rat kidney. / Bonhomme, Marie Chantal; Grove, Kevin; Caron, Sylvie; Crilley, Caroline T.; Thibault, Gaetan; Deschepper, Christian F.; Garcia, Raul.

In: Journal of the American Society of Nephrology, Vol. 9, No. 10, 10.1998, p. 1777-1786.

Research output: Contribution to journalArticle

Bonhomme, MC, Grove, K, Caron, S, Crilley, CT, Thibault, G, Deschepper, CF & Garcia, R 1998, 'Immunolocalization of natriuretic peptide receptor B in the rat kidney', Journal of the American Society of Nephrology, vol. 9, no. 10, pp. 1777-1786.
Bonhomme MC, Grove K, Caron S, Crilley CT, Thibault G, Deschepper CF et al. Immunolocalization of natriuretic peptide receptor B in the rat kidney. Journal of the American Society of Nephrology. 1998 Oct;9(10):1777-1786.
Bonhomme, Marie Chantal ; Grove, Kevin ; Caron, Sylvie ; Crilley, Caroline T. ; Thibault, Gaetan ; Deschepper, Christian F. ; Garcia, Raul. / Immunolocalization of natriuretic peptide receptor B in the rat kidney. In: Journal of the American Society of Nephrology. 1998 ; Vol. 9, No. 10. pp. 1777-1786.
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