Immunolocalization of basic fibroblast growth factor, fibroblast growth factor receptor-2, epidermal growth factor receptor and proliferating cell nuclear antigen in pterygia

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Abstract

Purpose. Basic fibroblast growth factor (bFGF) is a potent mitogenic peptide for a variety of cell types. Pterygium is a process in which fibrovascular tissue from the bulbar conjunctiva encroaches onto the cornea. This study was performed to determine if bFGF and its receptor are expressed in pterygia tissues. Epidermal growth factor receptor (EGF-R) was examined for comparison. Methods. Formalin fixed paraffin-embedded tissue samples of pterygia (N=12) were studied immunohistochemically with antibodies to bFGF, bFGF-R2 (bek), EGF-R and proliferating cell nuclear antigen (PCNA). 6 samples were doubled labeled with antibodies to bFGF and tryptase (a mast cell marker) to determine if mast cells are a source of bFGF in pterygia. Results. bFGF was immunolocalized in blood vessels, epithelium, and a subset of mononuclear cells. Strong immunostaining for FGF-R2 was seen in blood vessels and the epithelium. Intense immunostaining for EGF-R was seen in the epithelium but the subepithelial tissue was negative. PCNA was immunolocalized to epithelial cells and some endothelial cells in the subepithelial region. The majority of bFGF positive mononuclear cells were also tryptase positive (90.4±3.6%). Conclusions. These findings suggest that bFGF may contribute to the cellular proliferation in pterygia and mast cells are a source of this growth factor. The EGF-R results suggest that transforming growth factor-α or epidermal growth factor could also contribute to the epithelial cell proliferation in pterygia.

Original languageEnglish (US)
JournalInvestigative Ophthalmology and Visual Science
Volume37
Issue number3
StatePublished - Feb 15 1996

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Receptor, Fibroblast Growth Factor, Type 2
Pterygium
Proliferating Cell Nuclear Antigen
Fibroblast Growth Factor 2
Epidermal Growth Factor Receptor
Tryptases
Epithelium
Mast Cells
Blood Vessels
Epithelial Cells
Cell Proliferation
Fibroblast Growth Factor Receptors
Antibodies
Conjunctiva
Transforming Growth Factors
Epidermal Growth Factor
Paraffin
Cornea
Formaldehyde
Intercellular Signaling Peptides and Proteins

ASJC Scopus subject areas

  • Ophthalmology

Cite this

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title = "Immunolocalization of basic fibroblast growth factor, fibroblast growth factor receptor-2, epidermal growth factor receptor and proliferating cell nuclear antigen in pterygia",
abstract = "Purpose. Basic fibroblast growth factor (bFGF) is a potent mitogenic peptide for a variety of cell types. Pterygium is a process in which fibrovascular tissue from the bulbar conjunctiva encroaches onto the cornea. This study was performed to determine if bFGF and its receptor are expressed in pterygia tissues. Epidermal growth factor receptor (EGF-R) was examined for comparison. Methods. Formalin fixed paraffin-embedded tissue samples of pterygia (N=12) were studied immunohistochemically with antibodies to bFGF, bFGF-R2 (bek), EGF-R and proliferating cell nuclear antigen (PCNA). 6 samples were doubled labeled with antibodies to bFGF and tryptase (a mast cell marker) to determine if mast cells are a source of bFGF in pterygia. Results. bFGF was immunolocalized in blood vessels, epithelium, and a subset of mononuclear cells. Strong immunostaining for FGF-R2 was seen in blood vessels and the epithelium. Intense immunostaining for EGF-R was seen in the epithelium but the subepithelial tissue was negative. PCNA was immunolocalized to epithelial cells and some endothelial cells in the subepithelial region. The majority of bFGF positive mononuclear cells were also tryptase positive (90.4±3.6{\%}). Conclusions. These findings suggest that bFGF may contribute to the cellular proliferation in pterygia and mast cells are a source of this growth factor. The EGF-R results suggest that transforming growth factor-α or epidermal growth factor could also contribute to the epithelial cell proliferation in pterygia.",
author = "Thompson, {J. E.} and Powers, {Michael (Mike)} and B. O'Brien and David Wilson and Rosenbaum, {James (Jim)}",
year = "1996",
month = "2",
day = "15",
language = "English (US)",
volume = "37",
journal = "Investigative Ophthalmology and Visual Science",
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TY - JOUR

T1 - Immunolocalization of basic fibroblast growth factor, fibroblast growth factor receptor-2, epidermal growth factor receptor and proliferating cell nuclear antigen in pterygia

AU - Thompson, J. E.

AU - Powers, Michael (Mike)

AU - O'Brien, B.

AU - Wilson, David

AU - Rosenbaum, James (Jim)

PY - 1996/2/15

Y1 - 1996/2/15

N2 - Purpose. Basic fibroblast growth factor (bFGF) is a potent mitogenic peptide for a variety of cell types. Pterygium is a process in which fibrovascular tissue from the bulbar conjunctiva encroaches onto the cornea. This study was performed to determine if bFGF and its receptor are expressed in pterygia tissues. Epidermal growth factor receptor (EGF-R) was examined for comparison. Methods. Formalin fixed paraffin-embedded tissue samples of pterygia (N=12) were studied immunohistochemically with antibodies to bFGF, bFGF-R2 (bek), EGF-R and proliferating cell nuclear antigen (PCNA). 6 samples were doubled labeled with antibodies to bFGF and tryptase (a mast cell marker) to determine if mast cells are a source of bFGF in pterygia. Results. bFGF was immunolocalized in blood vessels, epithelium, and a subset of mononuclear cells. Strong immunostaining for FGF-R2 was seen in blood vessels and the epithelium. Intense immunostaining for EGF-R was seen in the epithelium but the subepithelial tissue was negative. PCNA was immunolocalized to epithelial cells and some endothelial cells in the subepithelial region. The majority of bFGF positive mononuclear cells were also tryptase positive (90.4±3.6%). Conclusions. These findings suggest that bFGF may contribute to the cellular proliferation in pterygia and mast cells are a source of this growth factor. The EGF-R results suggest that transforming growth factor-α or epidermal growth factor could also contribute to the epithelial cell proliferation in pterygia.

AB - Purpose. Basic fibroblast growth factor (bFGF) is a potent mitogenic peptide for a variety of cell types. Pterygium is a process in which fibrovascular tissue from the bulbar conjunctiva encroaches onto the cornea. This study was performed to determine if bFGF and its receptor are expressed in pterygia tissues. Epidermal growth factor receptor (EGF-R) was examined for comparison. Methods. Formalin fixed paraffin-embedded tissue samples of pterygia (N=12) were studied immunohistochemically with antibodies to bFGF, bFGF-R2 (bek), EGF-R and proliferating cell nuclear antigen (PCNA). 6 samples were doubled labeled with antibodies to bFGF and tryptase (a mast cell marker) to determine if mast cells are a source of bFGF in pterygia. Results. bFGF was immunolocalized in blood vessels, epithelium, and a subset of mononuclear cells. Strong immunostaining for FGF-R2 was seen in blood vessels and the epithelium. Intense immunostaining for EGF-R was seen in the epithelium but the subepithelial tissue was negative. PCNA was immunolocalized to epithelial cells and some endothelial cells in the subepithelial region. The majority of bFGF positive mononuclear cells were also tryptase positive (90.4±3.6%). Conclusions. These findings suggest that bFGF may contribute to the cellular proliferation in pterygia and mast cells are a source of this growth factor. The EGF-R results suggest that transforming growth factor-α or epidermal growth factor could also contribute to the epithelial cell proliferation in pterygia.

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