Immunolocalization of basic fibroblast growth factor and platelet-derived growth factor-A during adjuvant arthritis in the Lewis rat

Zhenhong Qu, Marcia Picou, Thao Tran Dang, Ethen Angell, Stephen Planck, Charles E. Hart, James (Jim) Rosenbaum

Research output: Contribution to journalArticle

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Abstract

A prerequisite in defining the role of a growth factor in a disease is knowledge of its expression kinetics during the natural course of the disease. We, therefore, used immunohistochemical and immunoblot analyses to examine tissue distribution of basic fibroblast growth factor (bFGF) and platelet-derived growth factor (PDGF-A) during the development of destructive arthropathy in the rat adjuvant arthritis model. In normal joints, bFGF was primarily localized in endothelial cells. In inflamed joints, increased staining for bFGF was found in the invading panni, hyperplastic synovium, and thickened periosteum where bFGF was also co-localized with two cell proliferation markers. Staining for bFGF began to increase at the onset of arthritis (days 11 to 13), reached peak level on days 17 to 24, and gradually declined afterward. In contrast, PDGF-A staining did not change until day 17 and the increased staining was restricted to areas of newly formed bone. The distinct temporal and spatial distribution pattern of these two growth factors during the destructive arthropathy strongly suggests that they play different roles during arthritis. Although PDGF-A seems to be exclusively related to osteogenesis, bFGF may have a more extensive impact on synovial proliferation and bone destruction as well as bone formation.

Original languageEnglish (US)
Pages (from-to)1127-1139
Number of pages13
JournalAmerican Journal of Pathology
Volume145
Issue number5
StatePublished - Nov 1994

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Experimental Arthritis
Fibroblast Growth Factor 2
Platelet-Derived Growth Factor
Staining and Labeling
Joint Diseases
Osteogenesis
Arthritis
Intercellular Signaling Peptides and Proteins
Joints
Bone and Bones
Periosteum
Synovial Membrane
Tissue Distribution
platelet-derived growth factor A
Endothelial Cells
Cell Proliferation

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

Cite this

Immunolocalization of basic fibroblast growth factor and platelet-derived growth factor-A during adjuvant arthritis in the Lewis rat. / Qu, Zhenhong; Picou, Marcia; Dang, Thao Tran; Angell, Ethen; Planck, Stephen; Hart, Charles E.; Rosenbaum, James (Jim).

In: American Journal of Pathology, Vol. 145, No. 5, 11.1994, p. 1127-1139.

Research output: Contribution to journalArticle

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