Immunohistochemical study of a chondroitin-6-sulfate in growth plates of broiler chickens with high and low genetic predispositions to tibial dyschondroplasia

Junxiu Ling, S. A. Kincaid, G. R. McDaniel, J. E. Barrels, Brian Johnstone

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

The distribution of a chondroitin-6-sulfate (C6S) epitope, which is a biochemical marker of chondrocyte hypertrophy, was studied in the growth plates of two lines of 3-week-old broiler chickens with low and high genetic predispositions to tibial dyschondroplasia (TD). Ultrathin sections of growth plates from both groups were subjected to immunolocalization with monoclonal antibody 3-B-3(-), the epitope of which is increased on proteoglycans made by hypertrophic chondrocytes. Bound antibody was localized with colloidal gold-labeled protein A for observation with an electron microscope. The 3-B-3(-) epitope was localized in pericellular and interterritorial matrix of growth plates of both lines. In the low-TD-incidence birds, the concentration of 3-B-3(-) bound to C6S progressively increased from the proliferative zone to the hypertrophic zone. However, in the high-TD-incidence line, the epitope expression remained at a low level in all zones. The increase of the 3-B-3(-) epitope produced by maturing growth-plate chondrocytes is indicative of changes in the glycosaminoglycan chains of proteoglycans that may be important in the process of matrical calcification. Thus, failure of chondrocytes of the high-TD-incidence line to produce this change in post-translational modification of their proteoglycans could be important in the pathological process.

Original languageEnglish (US)
Pages (from-to)88-98
Number of pages11
JournalAvian Diseases
Volume40
Issue number1
StatePublished - 1996
Externally publishedYes

Fingerprint

Osteochondrodysplasias
dyschondroplasia
growth plate
Growth Plate
Chondroitin Sulfates
Genetic Predisposition to Disease
epitopes
chondrocytes
Epitopes
Chickens
Chondrocytes
sulfates
broiler chickens
proteoglycans
Proteoglycans
incidence
Incidence
Gold Colloid
glycosaminoglycans
post-translational modification

ASJC Scopus subject areas

  • Cancer Research
  • Immunology
  • veterinary(all)

Cite this

Immunohistochemical study of a chondroitin-6-sulfate in growth plates of broiler chickens with high and low genetic predispositions to tibial dyschondroplasia. / Ling, Junxiu; Kincaid, S. A.; McDaniel, G. R.; Barrels, J. E.; Johnstone, Brian.

In: Avian Diseases, Vol. 40, No. 1, 1996, p. 88-98.

Research output: Contribution to journalArticle

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abstract = "The distribution of a chondroitin-6-sulfate (C6S) epitope, which is a biochemical marker of chondrocyte hypertrophy, was studied in the growth plates of two lines of 3-week-old broiler chickens with low and high genetic predispositions to tibial dyschondroplasia (TD). Ultrathin sections of growth plates from both groups were subjected to immunolocalization with monoclonal antibody 3-B-3(-), the epitope of which is increased on proteoglycans made by hypertrophic chondrocytes. Bound antibody was localized with colloidal gold-labeled protein A for observation with an electron microscope. The 3-B-3(-) epitope was localized in pericellular and interterritorial matrix of growth plates of both lines. In the low-TD-incidence birds, the concentration of 3-B-3(-) bound to C6S progressively increased from the proliferative zone to the hypertrophic zone. However, in the high-TD-incidence line, the epitope expression remained at a low level in all zones. The increase of the 3-B-3(-) epitope produced by maturing growth-plate chondrocytes is indicative of changes in the glycosaminoglycan chains of proteoglycans that may be important in the process of matrical calcification. Thus, failure of chondrocytes of the high-TD-incidence line to produce this change in post-translational modification of their proteoglycans could be important in the pathological process.",
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