Immunohistochemical staining of Langerhans cells and macrophages in oral lichen planus

Joseph A. Regezi, Jeffery Stewart, Ricardo V. Lloyd, John T. Headington

Research output: Contribution to journalArticle

39 Citations (Scopus)

Abstract

Twenty patients with clinically and microscopically confirmed lichen planus were studied immunohistochemically. Monoclonal antibody to HLA-DR antigens and polyclonal antisera to S-100 protein and muramidase were applied to paraffin-embedded sections for the purpose of elaborating on the pathogenesis of this disease. Trypsin incubation of sections was also done in order to determine its effect on immunostaining. Langerhans cells were identified with anti-S-100 and anti-HLA-DR, and macrophages were identified with antimuramidase and anti-HLA-DR. Keratinocytes also expressed HLA-DR membrane activity in lichen planus tissue. Trypsinization significantly improved the expression of S-100 protein and muramidase antigens. It was concluded that Langerhans cells, macrophages, and keratinocytes play important roles in antigen processing and/or phagocytosis during the natural history of this disease.

Original languageEnglish (US)
Pages (from-to)396-402
Number of pages7
JournalOral Surgery, Oral Medicine, Oral Pathology
Volume60
Issue number4
DOIs
StatePublished - 1985
Externally publishedYes

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Oral Lichen Planus
Langerhans Cells
HLA-DR Antigens
Macrophages
Staining and Labeling
Lichen Planus
S100 Proteins
Muramidase
Keratinocytes
Antigen Presentation
Phagocytosis
Paraffin
Trypsin
Immune Sera
Monoclonal Antibodies
Antigens
Membranes

ASJC Scopus subject areas

  • Pathology and Forensic Medicine
  • Dentistry(all)

Cite this

Immunohistochemical staining of Langerhans cells and macrophages in oral lichen planus. / Regezi, Joseph A.; Stewart, Jeffery; Lloyd, Ricardo V.; Headington, John T.

In: Oral Surgery, Oral Medicine, Oral Pathology, Vol. 60, No. 4, 1985, p. 396-402.

Research output: Contribution to journalArticle

Regezi, Joseph A. ; Stewart, Jeffery ; Lloyd, Ricardo V. ; Headington, John T. / Immunohistochemical staining of Langerhans cells and macrophages in oral lichen planus. In: Oral Surgery, Oral Medicine, Oral Pathology. 1985 ; Vol. 60, No. 4. pp. 396-402.
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