Immunohistochemical localization of the neuron-specific glucose transporter (GLUT3) to neuropil in adult rat brain

Anthony L. McCall; Antonia M. Van Bueren; Melissa Moholt-Siebert; Nicola J. Cherry; William R. Woodward

doi:10.1016/0006-8993(94)90896-6

Immunohistochemical localization of the neuron-specific glucose transporter (GLUT3) to neuropil in adult rat brain

Anthony L. McCall, Antonia M. Van Bueren, Melissa Moholt-Siebert, Nicola J. Cherry, William R. Woodward

Chemical Physiology and Biochemistry

Research output: Contribution to journal › Article › peer-review

78 Scopus citations

Abstract

The precise histologic localization of GLUT3, a glucose transporter thought to be restricted to neurons, is unknown. Using a high-affinity, specific antiserum against rodent GLUT3 for immunocytochemistry, light microscopic staining concentrates heterogeneously in the neuropil in a region- and lamina-specific manner; intense staining characterizes areas with high rates of glucose utilization such as inferior colliculus and pyriform cortex. Neuropil localization with little perikaryal staining suggests that GLUT3 may provide the energy needed locally for synaptic transmission.

Original language	English (US)
Pages (from-to)	292-297
Number of pages	6
Journal	Brain research
Volume	659
Issue number	1-2
DOIs	https://doi.org/10.1016/0006-8993(94)90896-6
State	Published - Oct 3 1994

Keywords

Brain metabolism
Energy metabolism
Glucose transporter
Immunocytochemistry
Synapse

ASJC Scopus subject areas

General Neuroscience
Molecular Biology
Clinical Neurology
Developmental Biology

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10.1016/0006-8993(94)90896-6

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@article{7871b320bc28401d90ba9764a24d8461,

title = "Immunohistochemical localization of the neuron-specific glucose transporter (GLUT3) to neuropil in adult rat brain",

abstract = "The precise histologic localization of GLUT3, a glucose transporter thought to be restricted to neurons, is unknown. Using a high-affinity, specific antiserum against rodent GLUT3 for immunocytochemistry, light microscopic staining concentrates heterogeneously in the neuropil in a region- and lamina-specific manner; intense staining characterizes areas with high rates of glucose utilization such as inferior colliculus and pyriform cortex. Neuropil localization with little perikaryal staining suggests that GLUT3 may provide the energy needed locally for synaptic transmission.",

keywords = "Brain metabolism, Energy metabolism, Glucose transporter, Immunocytochemistry, Synapse",

author = "McCall, {Anthony L.} and {Van Bueren}, {Antonia M.} and Melissa Moholt-Siebert and Cherry, {Nicola J.} and Woodward, {William R.}",

note = "Funding Information: We acknowledge helpful discussions with Dr. Phillip Schwartzkroin of the University of Washington regarding hippocampal GLUT3 staining. We thank Dr. Di-anne F. Lattemann for editorial suggestions. The mouse GLUT3 cDNA which we used to assess the specificity of our GLUT3 antiserum was kindly provided by Dr. Graeme Bell of the University of Chicago. This work was supported by grants in aid from the Department of Health and Human Services including NIH Grants NS22213 and NS17493.",

year = "1994",

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doi = "10.1016/0006-8993(94)90896-6",

language = "English (US)",

volume = "659",

pages = "292--297",

journal = "Brain research",

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AU - McCall, Anthony L.

AU - Van Bueren, Antonia M.

AU - Moholt-Siebert, Melissa

AU - Cherry, Nicola J.

AU - Woodward, William R.

N1 - Funding Information: We acknowledge helpful discussions with Dr. Phillip Schwartzkroin of the University of Washington regarding hippocampal GLUT3 staining. We thank Dr. Di-anne F. Lattemann for editorial suggestions. The mouse GLUT3 cDNA which we used to assess the specificity of our GLUT3 antiserum was kindly provided by Dr. Graeme Bell of the University of Chicago. This work was supported by grants in aid from the Department of Health and Human Services including NIH Grants NS22213 and NS17493.

PY - 1994/10/3

Y1 - 1994/10/3

N2 - The precise histologic localization of GLUT3, a glucose transporter thought to be restricted to neurons, is unknown. Using a high-affinity, specific antiserum against rodent GLUT3 for immunocytochemistry, light microscopic staining concentrates heterogeneously in the neuropil in a region- and lamina-specific manner; intense staining characterizes areas with high rates of glucose utilization such as inferior colliculus and pyriform cortex. Neuropil localization with little perikaryal staining suggests that GLUT3 may provide the energy needed locally for synaptic transmission.

AB - The precise histologic localization of GLUT3, a glucose transporter thought to be restricted to neurons, is unknown. Using a high-affinity, specific antiserum against rodent GLUT3 for immunocytochemistry, light microscopic staining concentrates heterogeneously in the neuropil in a region- and lamina-specific manner; intense staining characterizes areas with high rates of glucose utilization such as inferior colliculus and pyriform cortex. Neuropil localization with little perikaryal staining suggests that GLUT3 may provide the energy needed locally for synaptic transmission.

KW - Brain metabolism

KW - Energy metabolism

KW - Glucose transporter

KW - Immunocytochemistry

KW - Synapse

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Immunohistochemical localization of the neuron-specific glucose transporter (GLUT3) to neuropil in adult rat brain

Abstract

Keywords

ASJC Scopus subject areas

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