Immunohistochemical localization of phospholipase A2 isoforms in human myometrium during pregnancy and parturition

D. G. Skannal, A. L W Eis, D. Brockman, T. A. Siddiqi, Leslie Myatt

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

OBJECTIVE: The purpose of this study was to localize secretory/phospholipase A2 and cytosolic phospholipase A2 isoforms in pregnant human myometrium and to determine changes in expression with gestational age or parturition. STUDY DESIGN: Myometrium was collected at cesarean section at term (>37 weeks) or preterm (2 or cytosolic phospholipase A2 and immunostaining visualized with the Vectastain ABC method. The intensity of immunostaining in different cellular localizations was scored by an investigator blinded to tissue identity and compared among tissues with use of the Mantel-Haenszel χ2 test. RESULTS: Secretory phospholipase A2 immunostaining was dispersed in the perinuclear region throughout the myometrial smooth muscle fibers and in vascular smooth muscle. Cytosolic phospholipase A2 immunostaining was predominantly localized to endothelial cells of myometrial blood vessels and weakly throughout myometrial fibers. There was no apparent change in intensity of immunostaining for either isoform with gestational age or with the absence or presence of labor. CONCLUSION: The differential localization of the two phospholipase A2 isoforms suggests different functions. The apparent lack of change in expression during late gestation or with labor possibly suggests changes in myometrial phospholipase A2 activity and hence local myometrial arachidonic acid mobilization and presumably prostaglandin synthesis may not be associated with the onset of or maintenance of parturition.

Original languageEnglish (US)
Pages (from-to)878-882
Number of pages5
JournalAmerican Journal of Obstetrics and Gynecology
Volume176
Issue number4
StatePublished - 1997
Externally publishedYes

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Cytosolic Phospholipases A2
Myometrium
Phospholipases A2
Secretory Phospholipase A2
Protein Isoforms
Parturition
Pregnancy
Gestational Age
Vascular Smooth Muscle
Arachidonic Acid
Cesarean Section
Prostaglandins
Smooth Muscle
Blood Vessels
Endothelial Cells
Maintenance
Research Personnel

Keywords

  • Myometrium
  • parturition
  • phospholipase A

ASJC Scopus subject areas

  • Medicine(all)
  • Obstetrics and Gynecology

Cite this

Immunohistochemical localization of phospholipase A2 isoforms in human myometrium during pregnancy and parturition. / Skannal, D. G.; Eis, A. L W; Brockman, D.; Siddiqi, T. A.; Myatt, Leslie.

In: American Journal of Obstetrics and Gynecology, Vol. 176, No. 4, 1997, p. 878-882.

Research output: Contribution to journalArticle

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