Immunohistochemical identification and synaptic inputs to the diffuse bipolar cell type DB1 in macaque retina

Teresa Puthussery, Jacqueline Gayet-Primo, William Taylor, Silke Haverkamp

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

Detailed analysis of the synaptic inputs to the primate DB1 bipolar cell has been precluded by the absence of a suitable immunohistochemical marker. Here we demonstrate that antibodies for the EF-hand calcium-binding protein, secretagogin, strongly label the DB1 bipolar cell as well as a mixed population of GABAergic amacrine cells in the macaque retina. Using secretagogin as a marker, we show that the DB1 bipolar makes synaptic contact with both L/M as well as S-cone photoreceptors and only minimal contact with rod photoreceptors. Electron microscopy showed that the DB1 bipolar makes flat contacts at both triad-associated and nontriad-associated positions on the cone pedicle. Double labeling with various glutamate receptor subunit antibodies failed to conclusively determine the subunit composition of the glutamate receptors on DB1 bipolar cells. In the IPL, DB1 bipolar cell axon terminals expressed the glycine receptor, GlyRα1, at sites of contact with AII amacrine cells, suggesting that these cells receive input from the rod pathway.

Original languageEnglish (US)
Pages (from-to)3640-3656
Number of pages17
JournalJournal of Comparative Neurology
Volume519
Issue number18
DOIs
StatePublished - Dec 15 2011

Fingerprint

Macaca
Retina
Amacrine Cells
Glutamate Receptors
Retinal Cone Photoreceptor Cells
EF Hand Motifs
Glycine Receptors
Retinal Rod Photoreceptor Cells
Calcium-Binding Proteins
Antibodies
Presynaptic Terminals
Primates
Electron Microscopy
Population

Keywords

  • Amacrine cell
  • Blue cone input
  • Cone photoreceptors
  • Glutamate receptors
  • Glycine receptor
  • OFF cone bipolar cells
  • Secretagogin

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Immunohistochemical identification and synaptic inputs to the diffuse bipolar cell type DB1 in macaque retina. / Puthussery, Teresa; Gayet-Primo, Jacqueline; Taylor, William; Haverkamp, Silke.

In: Journal of Comparative Neurology, Vol. 519, No. 18, 15.12.2011, p. 3640-3656.

Research output: Contribution to journalArticle

Puthussery, Teresa ; Gayet-Primo, Jacqueline ; Taylor, William ; Haverkamp, Silke. / Immunohistochemical identification and synaptic inputs to the diffuse bipolar cell type DB1 in macaque retina. In: Journal of Comparative Neurology. 2011 ; Vol. 519, No. 18. pp. 3640-3656.
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