Immunohistochemical determination of the estrogen receptor content of gastrointestinal adenocarcinomas

B. L. Cameron, J. A. Butler, J. Rutgers, H. I. Vargas, M. Purtell, Brett Sheppard

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

Recently there have been several reports documenting the presence of estrogen receptors (ERs) in human gastrointestinal (GI) malignancies, raising the possibility that these cancers may be hormonally manipulated. To test this hypothesis, 68 frozen GI tissue specimens were examined for the presence of ERs within the cell nuclei by immunohistochemical staining. There were 51 cancers (25 gastric, 26 colon) and 17 normal tissue specimens (six gastric, 11 colon). Tissue sections 4 to 6 microns thick were incubated with monoclonal antibody H222SPφ, then stained by the peroxidase-antiperoxidase technique for visualization of the ER. The staining was semiquantitatively graded from 0 to 3+ depending on both the intensity of staining and the percentage of cell nuclei stained. A breast cancer specimen known to be strongly positive for the ER was used as a positive control. The 30 male and 21 female cancer patients had a median age of 59 years. All tumors were adenocarcinomas. Eight per cent of the gastric cancers were poorly differentiated, while 94 per cent of the colon cancers were moderately well to well differentiated. Using weak staining of at least 20 per cent of the cell nuclei as the minimum requirement for an ER positive tumor (1+, 0/51 tumors and 0/17) normal specimens were positive for ER. Only three out of 25 (12%) gastric tumors, and two out of 26 (8%) colon tumors demonstrated any staining; each exhibited weak staining of only five to ten per cent of the tumor nuclei. The authors conclude that ERs are found at minimally detectable levels in only a small percentage of gastric and colon cancers, and thus are unlikely to play a significant role in the growth of these tumors. If tamoxifen is effective in the treatment of these cancers, the mechanism is probably unrelated to binding of the estrogen receptor.

Original languageEnglish (US)
Pages (from-to)758-760
Number of pages3
JournalAmerican Surgeon
Volume58
Issue number12
StatePublished - 1992
Externally publishedYes

Fingerprint

Gastrointestinal Contents
Estrogen Receptors
Adenocarcinoma
Neoplasms
Staining and Labeling
Cell Nucleus
Stomach Neoplasms
Colon
Colonic Neoplasms
Stomach
Tamoxifen
Peroxidase
Monoclonal Antibodies
Breast Neoplasms

ASJC Scopus subject areas

  • Surgery

Cite this

Cameron, B. L., Butler, J. A., Rutgers, J., Vargas, H. I., Purtell, M., & Sheppard, B. (1992). Immunohistochemical determination of the estrogen receptor content of gastrointestinal adenocarcinomas. American Surgeon, 58(12), 758-760.

Immunohistochemical determination of the estrogen receptor content of gastrointestinal adenocarcinomas. / Cameron, B. L.; Butler, J. A.; Rutgers, J.; Vargas, H. I.; Purtell, M.; Sheppard, Brett.

In: American Surgeon, Vol. 58, No. 12, 1992, p. 758-760.

Research output: Contribution to journalArticle

Cameron, BL, Butler, JA, Rutgers, J, Vargas, HI, Purtell, M & Sheppard, B 1992, 'Immunohistochemical determination of the estrogen receptor content of gastrointestinal adenocarcinomas', American Surgeon, vol. 58, no. 12, pp. 758-760.
Cameron, B. L. ; Butler, J. A. ; Rutgers, J. ; Vargas, H. I. ; Purtell, M. ; Sheppard, Brett. / Immunohistochemical determination of the estrogen receptor content of gastrointestinal adenocarcinomas. In: American Surgeon. 1992 ; Vol. 58, No. 12. pp. 758-760.
@article{c2587240f6c04d0c8364f0724adeaffe,
title = "Immunohistochemical determination of the estrogen receptor content of gastrointestinal adenocarcinomas",
abstract = "Recently there have been several reports documenting the presence of estrogen receptors (ERs) in human gastrointestinal (GI) malignancies, raising the possibility that these cancers may be hormonally manipulated. To test this hypothesis, 68 frozen GI tissue specimens were examined for the presence of ERs within the cell nuclei by immunohistochemical staining. There were 51 cancers (25 gastric, 26 colon) and 17 normal tissue specimens (six gastric, 11 colon). Tissue sections 4 to 6 microns thick were incubated with monoclonal antibody H222SPφ, then stained by the peroxidase-antiperoxidase technique for visualization of the ER. The staining was semiquantitatively graded from 0 to 3+ depending on both the intensity of staining and the percentage of cell nuclei stained. A breast cancer specimen known to be strongly positive for the ER was used as a positive control. The 30 male and 21 female cancer patients had a median age of 59 years. All tumors were adenocarcinomas. Eight per cent of the gastric cancers were poorly differentiated, while 94 per cent of the colon cancers were moderately well to well differentiated. Using weak staining of at least 20 per cent of the cell nuclei as the minimum requirement for an ER positive tumor (1+, 0/51 tumors and 0/17) normal specimens were positive for ER. Only three out of 25 (12{\%}) gastric tumors, and two out of 26 (8{\%}) colon tumors demonstrated any staining; each exhibited weak staining of only five to ten per cent of the tumor nuclei. The authors conclude that ERs are found at minimally detectable levels in only a small percentage of gastric and colon cancers, and thus are unlikely to play a significant role in the growth of these tumors. If tamoxifen is effective in the treatment of these cancers, the mechanism is probably unrelated to binding of the estrogen receptor.",
author = "Cameron, {B. L.} and Butler, {J. A.} and J. Rutgers and Vargas, {H. I.} and M. Purtell and Brett Sheppard",
year = "1992",
language = "English (US)",
volume = "58",
pages = "758--760",
journal = "The American surgeon",
issn = "0003-1348",
publisher = "Southeastern Surgical Congress",
number = "12",

}

TY - JOUR

T1 - Immunohistochemical determination of the estrogen receptor content of gastrointestinal adenocarcinomas

AU - Cameron, B. L.

AU - Butler, J. A.

AU - Rutgers, J.

AU - Vargas, H. I.

AU - Purtell, M.

AU - Sheppard, Brett

PY - 1992

Y1 - 1992

N2 - Recently there have been several reports documenting the presence of estrogen receptors (ERs) in human gastrointestinal (GI) malignancies, raising the possibility that these cancers may be hormonally manipulated. To test this hypothesis, 68 frozen GI tissue specimens were examined for the presence of ERs within the cell nuclei by immunohistochemical staining. There were 51 cancers (25 gastric, 26 colon) and 17 normal tissue specimens (six gastric, 11 colon). Tissue sections 4 to 6 microns thick were incubated with monoclonal antibody H222SPφ, then stained by the peroxidase-antiperoxidase technique for visualization of the ER. The staining was semiquantitatively graded from 0 to 3+ depending on both the intensity of staining and the percentage of cell nuclei stained. A breast cancer specimen known to be strongly positive for the ER was used as a positive control. The 30 male and 21 female cancer patients had a median age of 59 years. All tumors were adenocarcinomas. Eight per cent of the gastric cancers were poorly differentiated, while 94 per cent of the colon cancers were moderately well to well differentiated. Using weak staining of at least 20 per cent of the cell nuclei as the minimum requirement for an ER positive tumor (1+, 0/51 tumors and 0/17) normal specimens were positive for ER. Only three out of 25 (12%) gastric tumors, and two out of 26 (8%) colon tumors demonstrated any staining; each exhibited weak staining of only five to ten per cent of the tumor nuclei. The authors conclude that ERs are found at minimally detectable levels in only a small percentage of gastric and colon cancers, and thus are unlikely to play a significant role in the growth of these tumors. If tamoxifen is effective in the treatment of these cancers, the mechanism is probably unrelated to binding of the estrogen receptor.

AB - Recently there have been several reports documenting the presence of estrogen receptors (ERs) in human gastrointestinal (GI) malignancies, raising the possibility that these cancers may be hormonally manipulated. To test this hypothesis, 68 frozen GI tissue specimens were examined for the presence of ERs within the cell nuclei by immunohistochemical staining. There were 51 cancers (25 gastric, 26 colon) and 17 normal tissue specimens (six gastric, 11 colon). Tissue sections 4 to 6 microns thick were incubated with monoclonal antibody H222SPφ, then stained by the peroxidase-antiperoxidase technique for visualization of the ER. The staining was semiquantitatively graded from 0 to 3+ depending on both the intensity of staining and the percentage of cell nuclei stained. A breast cancer specimen known to be strongly positive for the ER was used as a positive control. The 30 male and 21 female cancer patients had a median age of 59 years. All tumors were adenocarcinomas. Eight per cent of the gastric cancers were poorly differentiated, while 94 per cent of the colon cancers were moderately well to well differentiated. Using weak staining of at least 20 per cent of the cell nuclei as the minimum requirement for an ER positive tumor (1+, 0/51 tumors and 0/17) normal specimens were positive for ER. Only three out of 25 (12%) gastric tumors, and two out of 26 (8%) colon tumors demonstrated any staining; each exhibited weak staining of only five to ten per cent of the tumor nuclei. The authors conclude that ERs are found at minimally detectable levels in only a small percentage of gastric and colon cancers, and thus are unlikely to play a significant role in the growth of these tumors. If tamoxifen is effective in the treatment of these cancers, the mechanism is probably unrelated to binding of the estrogen receptor.

UR - http://www.scopus.com/inward/record.url?scp=0027049037&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0027049037&partnerID=8YFLogxK

M3 - Article

C2 - 1456602

AN - SCOPUS:0027049037

VL - 58

SP - 758

EP - 760

JO - The American surgeon

JF - The American surgeon

SN - 0003-1348

IS - 12

ER -