Immunohistochemical detection of progestin receptors in hypothalamic β-endorphin and substance P neurons of steroid-treated monkeys

Cynthia Bethea, Andrea A. Widmann

    Research output: Contribution to journalArticle

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    Abstract

    Progesterone (P) acts in the central nervous system to increase prolactin secretion in estrogen (E)-primed female monkeys. β-Endorphin (BE) and Substance P (SP) are two hypothalamic peptides which increase prolactin secretion when administered to rats and monkeys. Studies were performed to determine if P acts on these two potential prolactin-releasing systems. The presence of a nuclear steroid receptor defines the cell as a target for the cognate hormone. Therefore, the hypothalamic populations of BE and SP neurons were examined for the presence and regulation of nuclear progestin receptors (PR) in spayed, E-treated (28 days) and E + P-treated monkeys (14 days E + 14 days E + P). Hypothalamic blocks were prepared after perfusion fixation with 4% paraformaldehyde. Cryosectioning (10 μm) was followed by double immunocytochemistry (ICC) for PR (black nuclear stain) and either BE or SP (brown cytoplasmic stain). Sections were processed for ICC at 100- or 200-μm intervals through the hypothalamic block. Peptidergic neurons with and without PR were counted in each section. The E + P-treated monkeys exhibited a significant increase in serum prolactin. BE neurons were found only in the arcuate nucleus (ARC) and median eminence (ME). The colocalization of BE and PR equaled 2% in spayed controls, 21% in the E-treated group and 25% in the E + P-treated group. SP neurons were located in a dorsomedial hypothalamic (DMH) subpopulation which extended caudally under the mamillary nuclei and in a subpopulation located in the ARC and ME. Neither the DMH or submamillary SP neurons contained PR. The percent colocalization of SP and PR in the ARC/ME equaled 5, 26 and 10% in the spayed, E- and E + P-treated groups, respectively. The decrease in PR + SP colocalization with P treatment is probably due to a decrease in SP and not to a decrease in PR immunoreactivity. In summary, E treatment induced PR in BE and SP neurons. Addition of P to the E treatment did not alter the expression of PR in BE neurons, but PR colocalization decreased in SP neurons. Therefore, it is unlikely that SP neurons could transduce the action of P on prolactin secretion in primates, but BE neurons may play an intermediary role.

    Original languageEnglish (US)
    Pages (from-to)132-141
    Number of pages10
    JournalNeuroendocrinology
    Volume63
    Issue number2
    StatePublished - Feb 1996

    Fingerprint

    Endorphins
    Progesterone Receptors
    Substance P
    Haplorhini
    Steroids
    Neurons
    Prolactin
    Median Eminence
    Arcuate Nucleus of Hypothalamus
    Cytoplasmic and Nuclear Receptors
    Coloring Agents
    Cryoultramicrotomy
    Immunohistochemistry
    Neurokinin-1 Receptors
    Steroid Receptors
    Primates
    Progesterone
    Estrogens
    Therapeutics
    Central Nervous System

    Keywords

    • β-endorphin
    • Gonadal steroid receptors
    • Gonadal steroids
    • Primates
    • Substance P

    ASJC Scopus subject areas

    • Endocrinology
    • Neuroscience(all)

    Cite this

    Immunohistochemical detection of progestin receptors in hypothalamic β-endorphin and substance P neurons of steroid-treated monkeys. / Bethea, Cynthia; Widmann, Andrea A.

    In: Neuroendocrinology, Vol. 63, No. 2, 02.1996, p. 132-141.

    Research output: Contribution to journalArticle

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