Immunogenicity in mice of tandem repeats of an epitope from herpes simplex gD protein when expressed by recombinant adenovirus vectors

Bojian Zheng, Frank L. Graham, David Johnson, Tomas Hanke, Mark R. McDermott, Ludvik Prevec

Research output: Contribution to journalArticle

35 Citations (Scopus)

Abstract

The antigenic and immunogenic potential was examined of human adenovirus type 5 (Ad) recombinants carrying and expressing from one to four tandem repeats of a linear neutralizing epitope from the gD protein of herpes simplex virus type 1 (HSV-1) as a fusion with the β-galactosidase protein. The fusion proteins produced by these Ad vectors in infected cell culture reacted with a herpes simplex virus (HSV) epitope-specific monoclonal antibody to a degree dependent on the number of epitope repeats in the protein. Mice immunized by intraperitoneal injection of the Ad vectors developed an anti-HSV immune response as measured by ELISA and by HSV-1 neutralization assays. The mean antibody titre induced by a single injection of the Ad vector increased with the number of epitope repeats expressed by the recombinant. Any animal that had developed a serum-neutralizing titre of at least 1:80 survived challenge with a normally lethal dose of HSV-2 administered by the intraperitoneal route. Recombinant vectors expressing four repeats of the HSV epitope were as effective in antibody induction and protection as an adenovirus vector carrying and expressing the entire HSV gD protein. These results suggest that the expression of tandem repeats of appropriate epitopic sequences by adenovirus vectors may provide a safe and effective method of immunizing against HSV infection.

Original languageEnglish (US)
Pages (from-to)1191-1198
Number of pages8
JournalVaccine
Volume11
Issue number12
DOIs
StatePublished - 1993
Externally publishedYes

Fingerprint

herpes simplex
Herpes Simplex
Tandem Repeat Sequences
tandem repeat sequences
Adenoviridae
Simplexvirus
epitopes
Epitopes
immune response
viruses
mice
Human herpesvirus 1
Human Herpesvirus 1
Proteins
proteins
neutralization
Galactosidases
Human herpesvirus 2
galactosidases
Human Adenoviruses

Keywords

  • epitope repeats
  • Herpes simplex virus type 1
  • immunogenicity
  • recombinant adenovirus vector

ASJC Scopus subject areas

  • Immunology
  • Microbiology
  • Virology
  • Infectious Diseases
  • Public Health, Environmental and Occupational Health
  • veterinary(all)

Cite this

Immunogenicity in mice of tandem repeats of an epitope from herpes simplex gD protein when expressed by recombinant adenovirus vectors. / Zheng, Bojian; Graham, Frank L.; Johnson, David; Hanke, Tomas; McDermott, Mark R.; Prevec, Ludvik.

In: Vaccine, Vol. 11, No. 12, 1993, p. 1191-1198.

Research output: Contribution to journalArticle

Zheng, Bojian ; Graham, Frank L. ; Johnson, David ; Hanke, Tomas ; McDermott, Mark R. ; Prevec, Ludvik. / Immunogenicity in mice of tandem repeats of an epitope from herpes simplex gD protein when expressed by recombinant adenovirus vectors. In: Vaccine. 1993 ; Vol. 11, No. 12. pp. 1191-1198.
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