Immunofluorescent characteristics of the diabetic cornea

J. S. Weiss, D. N. Sang, Daniel Albert

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

In view of the increasing awareness of corneal abnormalities in diabetes, five diabetic and five nondiabetic postmortem corneas were investigated. Indirect fluorescent antibody techniques were used for the detection of immunoglobulin, complement components, fibrinogen, and fibronectin, as some of these had already been found in the kidney, pancreas, and skin of diabetic patients. There was no obvious difference in the deposition of IgG, IgA, IgM, fibrinogen, or fibronectin between diabetic and control corneal specimens. Five diabetic specimens versus three control specimens demonstrated positive staining at 1:50 dilution for complement components Clq, C3, and C4. This staining was primarily present in the epithelium. Of greater interest was the finding that complement components were present in the basement membrane of two diabetic patients, but were not found in nondiabetic patients. In conclusion, diabetic corneas demonstrate unusual staining for complement components in the epithelial basement membrane.

Original languageEnglish (US)
Pages (from-to)131-138
Number of pages8
JournalCornea
Volume9
Issue number2
StatePublished - Jan 1 1990
Externally publishedYes

Fingerprint

Cornea
Staining and Labeling
Fibronectins
Basement Membrane
Fibrinogen
Indirect Fluorescent Antibody Technique
Immunoglobulin A
Immunoglobulin M
Immunoglobulins
Pancreas
Epithelium
Immunoglobulin G
Kidney
Skin

ASJC Scopus subject areas

  • Ophthalmology

Cite this

Weiss, J. S., Sang, D. N., & Albert, D. (1990). Immunofluorescent characteristics of the diabetic cornea. Cornea, 9(2), 131-138.

Immunofluorescent characteristics of the diabetic cornea. / Weiss, J. S.; Sang, D. N.; Albert, Daniel.

In: Cornea, Vol. 9, No. 2, 01.01.1990, p. 131-138.

Research output: Contribution to journalArticle

Weiss, JS, Sang, DN & Albert, D 1990, 'Immunofluorescent characteristics of the diabetic cornea', Cornea, vol. 9, no. 2, pp. 131-138.
Weiss, J. S. ; Sang, D. N. ; Albert, Daniel. / Immunofluorescent characteristics of the diabetic cornea. In: Cornea. 1990 ; Vol. 9, No. 2. pp. 131-138.
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