Immunofluorescence analysis of T-cell responses in health and disease

Holden T. Maecker, Vernon C. Maino, Louis Picker

Research output: Contribution to journalArticle

39 Citations (Scopus)

Abstract

The use of flow cytometry to study the functional responses of T cells by immunofluorescent staining for intracellular cytokines and other markers is a growing field of clinical interest. In this article, we describe methods for the rapid evaluation of T-cell responses to mitogens and specific antigens and explore how these assays might be valuable in various clinical settings.

Original languageEnglish (US)
Pages (from-to)391-399
Number of pages9
JournalJournal of Clinical Immunology
Volume20
Issue number6
DOIs
StatePublished - 2000

Fingerprint

Fluorescent Antibody Technique
T-Lymphocytes
Health
Mitogens
Flow Cytometry
Staining and Labeling
Cytokines
Antigens

Keywords

  • Clinical trials
  • Flow cytometry
  • Human
  • Intracellular cytokine
  • T cells

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

Cite this

Immunofluorescence analysis of T-cell responses in health and disease. / Maecker, Holden T.; Maino, Vernon C.; Picker, Louis.

In: Journal of Clinical Immunology, Vol. 20, No. 6, 2000, p. 391-399.

Research output: Contribution to journalArticle

Maecker, Holden T. ; Maino, Vernon C. ; Picker, Louis. / Immunofluorescence analysis of T-cell responses in health and disease. In: Journal of Clinical Immunology. 2000 ; Vol. 20, No. 6. pp. 391-399.
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