Immunofluorescence analysis of T-cell responses in health and disease

Holden T. Maecker, Vernon C. Maino, Louis J. Picker

Research output: Contribution to journalArticle

39 Scopus citations

Abstract

The use of flow cytometry to study the functional responses of T cells by immunofluorescent staining for intracellular cytokines and other markers is a growing field of clinical interest. In this article, we describe methods for the rapid evaluation of T-cell responses to mitogens and specific antigens and explore how these assays might be valuable in various clinical settings.

Original languageEnglish (US)
Pages (from-to)391-399
Number of pages9
JournalJournal of Clinical Immunology
Volume20
Issue number6
DOIs
StatePublished - Dec 1 2000

Keywords

  • Clinical trials
  • Flow cytometry
  • Human
  • Intracellular cytokine
  • T cells

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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