Immunodominant antigens of Actinobacillus actinomycetemcomitans serotypes a and c in high‐responder patients

Joseph V. Califano, Harvey A. Schenkein, John G. Tew

Research output: Contribution to journalArticlepeer-review

27 Scopus citations


This study was undertaken to examine the characteristics of the immunodominant antigens of Actinobacillus actinomycetemcomitans serotypes a and c. The top responders for A. actinomycetemcomitans serotypes a and c were selected (19 for serotype a and 21 for serotype c) from 150 clinically characterized patients. Competition assays revealed that 9 of 19 of these patients were reacting specifically to serotype a and 12 of 21 for serotype c. Limiting dilution analysis on Western blots revealed that most antigen bands apparent at low dilution disappeared as the patient's serum was diluted. The antigen band(s) remaining at the endpoint or the dilution corresponding to the antibody liter were defined as immunodominant. For serotype a there were several different Immunodominant antigens but none was present in more than half of the subjects. For serotype c the immunodominant antigens included a number of discrete bands and a diffuse smeared polysaccharide band. Only 2 of these antigens were present in the majority of the high‐responders: 92% had the smeared antigen and 67% had a 15 kDa antigen. The 15 kDa band was a protein common to all A. actinomycetemcomitans serotypes. The smeared antigen was unaffected by protease K treatment and gave a reaction of identity with the serotype c specific rabbit antiserum. This rabbit antiserum is specific for a mannan carbohydrate and does not react with LPS (23). Therefore, the smeared immunodominant antigen appears to be a polysaccharide containing mannan.

Original languageEnglish (US)
Pages (from-to)228-235
Number of pages8
JournalOral microbiology and immunology
Issue number4
StatePublished - Aug 1991
Externally publishedYes


  • Actinobacillus actinomycetemcomitans
  • antibody
  • periodontitis
  • serotype

ASJC Scopus subject areas

  • Microbiology
  • Immunology
  • Dentistry(all)
  • Microbiology (medical)


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