Immunodominant antigens of Actinobacillus actinomycetemcomitans serotypes a and c in high-responder patients.

Joseph Califano, H. A. Schenkein, J. G. Tew

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

This study was undertaken to examine the characteristics of the immunodominant antigens of Actinobacillus actinomycetemcomitans serotypes a and c. The top responders for A. actinomycetemcomitans serotypes a and c were selected (19 for serotype a and 21 for serotype c) from 150 clinically characterized patients. Competition assays revealed that 9 of 19 of these patients were reacting specifically to serotype a and 12 of 21 for serotype c. Limiting dilution analysis on Western blots revealed that most antigen bands apparent at low dilution disappeared as the patient's serum was diluted. The antigen band(s) remaining at the endpoint or the dilution corresponding to the antibody titer were defined as immunodominant. For serotype a there were several different immunodominant antigens but none was present in more than half of the subjects. For serotype c the immunodominant antigens included a number of discrete bands and a diffuse smeared polysaccharide band. Only 2 of these antigens were present in the majority of the high-responders: 92% had the smeared antigen and 67% had a 15 kDa antigen. The 15 kDa band was a protein common to all A. actinomycetemcomitans serotypes. The smeared antigen was unaffected by protease K treatment and gave a reaction of identity with the serotype c specific rabbit antiserum. This rabbit antiserum is specific for a mannan carbohydrate and does not react with LPS (23). Therefore, the smeared immunodominant antigen appears to be a polysaccharide containing mannan.

Original languageEnglish (US)
Pages (from-to)228-235
Number of pages8
JournalOral Microbiology and Immunology
Volume6
Issue number4
StatePublished - Aug 1991
Externally publishedYes

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Aggregatibacter actinomycetemcomitans
Immunodominant Epitopes
Antigens
Mannans
Polysaccharides
Immune Sera
Serogroup
Rabbits
Endopeptidase K
Western Blotting
Carbohydrates

ASJC Scopus subject areas

  • Immunology
  • Microbiology (medical)
  • Dentistry(all)

Cite this

Immunodominant antigens of Actinobacillus actinomycetemcomitans serotypes a and c in high-responder patients. / Califano, Joseph; Schenkein, H. A.; Tew, J. G.

In: Oral Microbiology and Immunology, Vol. 6, No. 4, 08.1991, p. 228-235.

Research output: Contribution to journalArticle

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