Immunodissection of the connective tissue matrix in human skin

Douglas R. Keene, M. Peter Marinkovich, Lynn Sakai

Research output: Contribution to journalArticle

35 Citations (Scopus)

Abstract

Much of what has been learned of the components and structure of human skin over the past few years has been accomplished with the aid of antibody technology. Antibodies are used in techniques such as affinity chromatography to isolate individual molecules and by immunofluorescence and immunoelectron microscopy to identify each of those molecules as components of specific macromolecular assemblies present within the dermis. This manuscript is meant not as a review of technique but instead as a summary of recent progress made in the understanding of dermal matrix architecture.

Original languageEnglish (US)
Pages (from-to)394-406
Number of pages13
JournalMicroscopy Research and Technique
Volume38
Issue number4
DOIs
StatePublished - Sep 18 1997
Externally publishedYes

Fingerprint

connective tissue
antibodies
skin (animal)
Antibodies
connective tissues
Connective Tissue
Skin
Tissue
Affinity chromatography
Molecules
Immunoelectron Microscopy
dermis
chromatography
Dermis
affinity chromatography
matrices
Affinity Chromatography
Fluorescence Microscopy
assemblies
fluorescent antibody technique

Keywords

  • Basement membrane
  • Collagen
  • Dermis
  • Elastic fiber
  • Epidermis
  • Fibrillin
  • Human skin
  • Microfibrils

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Anatomy
  • Instrumentation

Cite this

Immunodissection of the connective tissue matrix in human skin. / Keene, Douglas R.; Marinkovich, M. Peter; Sakai, Lynn.

In: Microscopy Research and Technique, Vol. 38, No. 4, 18.09.1997, p. 394-406.

Research output: Contribution to journalArticle

Keene, Douglas R. ; Marinkovich, M. Peter ; Sakai, Lynn. / Immunodissection of the connective tissue matrix in human skin. In: Microscopy Research and Technique. 1997 ; Vol. 38, No. 4. pp. 394-406.
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