Immunocytochemical studies of substance P and met-enkephalin in the basal ganglia and substantia nigra in huntington’s, parkinson’s and alzheimer’s diseases

Marjorie Grafe, Lysia S. Forno, Lawrence F. Eng

Research output: Contribution to journalArticle

75 Citations (Scopus)

Abstract

Immunocytochemical studies of the distribution and intensity of Substance P and Met-enkephalin staining in the basal ganglia and substantia nigra were carried out in five cases each of brains from patients with Huntington’s disease, Parkinson’s disease, Alzheimer’s disease, and normal controls. The usefulness of the peroxidase-antiperoxidase method for human autopsy material was confirmed. Substance P and Met-enkephalin fibers were distributed in essentially the same pattern as described in experimental animals and in human brains. In Huntington’s disease brains decreased Substance P staining was found in the internal globus pallidus and the substantia nigra, in agreement with radioimmunoassay studies by others. Met- enkephalin staining in the external globus pallidus was of normal intensity, although present within a shrunken area. In Parkinson’s and Alzheimer’s diseases there was intense immunoreactivity for Substance P in the globus pallidus and substantia nigra, and for Met-enkephalin in the globus pallidus, at variance with reported decreases in Parkinson’s disease by radioimmunoassay, but in essential agreement with other immunocytochemical studies. Immunocytochemical methods complement radioimmunoassays of human brain and may help in mapping neuropeptidergic pathways and in pinpointing abnormalities in these pathways in basal ganglia disorders.

Original languageEnglish (US)
Pages (from-to)47-59
Number of pages13
JournalJournal of Neuropathology and Experimental Neurology
Volume44
Issue number1
StatePublished - 1985
Externally publishedYes

Fingerprint

Methionine Enkephalin
Globus Pallidus
Huntington Disease
Substantia Nigra
Substance P
Basal Ganglia
Parkinson Disease
Alzheimer Disease
Radioimmunoassay
Brain
Staining and Labeling
Basal Ganglia Diseases
Peroxidase
Autopsy

Keywords

  • Basal ganglia
  • Endorphins
  • Huntington’s chorea
  • Immunocytochemistry
  • Met-enkephalin
  • Parkinson disease
  • Substance P

ASJC Scopus subject areas

  • Clinical Neurology
  • Pathology and Forensic Medicine
  • Cellular and Molecular Neuroscience
  • Neurology
  • Neuroscience(all)

Cite this

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title = "Immunocytochemical studies of substance P and met-enkephalin in the basal ganglia and substantia nigra in huntington’s, parkinson’s and alzheimer’s diseases",
abstract = "Immunocytochemical studies of the distribution and intensity of Substance P and Met-enkephalin staining in the basal ganglia and substantia nigra were carried out in five cases each of brains from patients with Huntington’s disease, Parkinson’s disease, Alzheimer’s disease, and normal controls. The usefulness of the peroxidase-antiperoxidase method for human autopsy material was confirmed. Substance P and Met-enkephalin fibers were distributed in essentially the same pattern as described in experimental animals and in human brains. In Huntington’s disease brains decreased Substance P staining was found in the internal globus pallidus and the substantia nigra, in agreement with radioimmunoassay studies by others. Met- enkephalin staining in the external globus pallidus was of normal intensity, although present within a shrunken area. In Parkinson’s and Alzheimer’s diseases there was intense immunoreactivity for Substance P in the globus pallidus and substantia nigra, and for Met-enkephalin in the globus pallidus, at variance with reported decreases in Parkinson’s disease by radioimmunoassay, but in essential agreement with other immunocytochemical studies. Immunocytochemical methods complement radioimmunoassays of human brain and may help in mapping neuropeptidergic pathways and in pinpointing abnormalities in these pathways in basal ganglia disorders.",
keywords = "Basal ganglia, Endorphins, Huntington’s chorea, Immunocytochemistry, Met-enkephalin, Parkinson disease, Substance P",
author = "Marjorie Grafe and Forno, {Lysia S.} and Eng, {Lawrence F.}",
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AU - Forno, Lysia S.

AU - Eng, Lawrence F.

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N2 - Immunocytochemical studies of the distribution and intensity of Substance P and Met-enkephalin staining in the basal ganglia and substantia nigra were carried out in five cases each of brains from patients with Huntington’s disease, Parkinson’s disease, Alzheimer’s disease, and normal controls. The usefulness of the peroxidase-antiperoxidase method for human autopsy material was confirmed. Substance P and Met-enkephalin fibers were distributed in essentially the same pattern as described in experimental animals and in human brains. In Huntington’s disease brains decreased Substance P staining was found in the internal globus pallidus and the substantia nigra, in agreement with radioimmunoassay studies by others. Met- enkephalin staining in the external globus pallidus was of normal intensity, although present within a shrunken area. In Parkinson’s and Alzheimer’s diseases there was intense immunoreactivity for Substance P in the globus pallidus and substantia nigra, and for Met-enkephalin in the globus pallidus, at variance with reported decreases in Parkinson’s disease by radioimmunoassay, but in essential agreement with other immunocytochemical studies. Immunocytochemical methods complement radioimmunoassays of human brain and may help in mapping neuropeptidergic pathways and in pinpointing abnormalities in these pathways in basal ganglia disorders.

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