Immunocytochemical localization of androgen receptors in brains of developing and adult male rhesus monkeys

Jerome V A Choate, Ov Slayden, John A. Resko

    Research output: Contribution to journalArticle

    41 Citations (Scopus)

    Abstract

    We localized immunoreactive androgen receptors in the central nervous system (CNS) of fetal and adult male rhesus macaques by immunocytochemisty using an affinity-purified polyclonal antibody to the first 21 amino acids of the human androgen receptor (AR). This antibody caused a shift in the mobility of AR-bound 3H-DHT on a sucrose gradient and recognized a protein of approx 116 kDa on Western blot. Other criteria for specificity are presented. We localized AR in the diencephalon of male rhesus monkey fetuses. Immunoreactive neurons were found in the medial hypothalamic area and the ventromedial nucleus of the hypothalamus on days 47, 61, and 124 of gestation. At 124 d of gestation, AR immunoreactivity was also found in the arcuate nucleus. AR immunostaining was not found in other diencephalic structures in fetal life, including the preoptic area. In the adult monkey, neurons in ventromedial, dorsomedial, and arcuate nuclei of the hypothalamus; cortical, medial, and accessory basal nuclei of the amygdala; and regions of the hippocampus and the anterior pituitary gland contained immunoreactive AR. These data indicate that AR is found in specific areas of the CNS early in fetal development, but they also appear in other brain areas as the fetus grows. At 124 d of gestation (term, 167 d), the hypothalamic location of immunoreactive AR is similar to the adult.

    Original languageEnglish (US)
    Pages (from-to)51-60
    Number of pages10
    JournalEndocrine
    Volume8
    Issue number1
    DOIs
    StatePublished - 1998

    Fingerprint

    Androgen Receptors
    Macaca mulatta
    Brain
    Arcuate Nucleus of Hypothalamus
    Fetus
    Pregnancy
    Central Nervous System
    Ventromedial Hypothalamic Nucleus
    Mediodorsal Thalamic Nucleus
    Neurons
    Diencephalon
    Anterior Pituitary Gland
    Preoptic Area
    Antibodies
    Fetal Development
    Hypothalamus
    Haplorhini
    Sucrose
    Hippocampus
    Western Blotting

    Keywords

    • Androgen receptor
    • Brain
    • Fetus
    • Immunocytochemistry
    • Primate

    ASJC Scopus subject areas

    • Endocrinology

    Cite this

    Immunocytochemical localization of androgen receptors in brains of developing and adult male rhesus monkeys. / Choate, Jerome V A; Slayden, Ov; Resko, John A.

    In: Endocrine, Vol. 8, No. 1, 1998, p. 51-60.

    Research output: Contribution to journalArticle

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