Immune deficiency following thermal trauma is associated with apoptotic cell death

J. A. Teodorczyk-Injeyan, M. Cembrzynska-Nowak, S. Lalani, W. J. Peters, Gordon Mills

Research output: Contribution to journalArticle

61 Citations (Scopus)

Abstract

Thermal injury-associated specific immune deficiency occurs despite indicators of systemic activation of the lymphoid compartment. We investigated the possibility that postburn immune failure and T cell activation are causally related through activation-induced (apoptotic) cell death. The relationship between the cellular immune response and cell mortality was examined in cultures of peripheral blood mononuclear cells (PBMC) from 14 immunosuppressed patients with extensive burns (35-90% total body surface area). Impaired cellular immunity coincided with significantly reduced cell viability as ascertained by propidium iodide staining and dye reduction assays. Following stimulation with the mitogenic lectin, phytohemagglutinin (PHA), the majority of DNA in patient cultures was fragmented, suggesting the occurrence of apoptotic cell death. Even without stimulation a portion of patient cells was apoptotic as indicated by oligonucleosomal bands on agarose gel electrophoresis. Exogenous interleukin-2 or phorbol ester markedly reduced constitutive as well as PHAinduced DNA fragmentation. In situ demonstration of DNA strand breaks in freshly isolated patient PBMC, by a TdT-based labeling technique, confirmed that a larger fraction (up to 60%) of circulating lymphocytes was undergoing apoptosis on the periphery. These novel observations suggest that apoptosis may play a major role in thermal injury-related cellular immunodeficiency.

Original languageEnglish (US)
Pages (from-to)318-328
Number of pages11
JournalJournal of Clinical Immunology
Volume15
Issue number6
DOIs
StatePublished - Nov 1 1995
Externally publishedYes

Fingerprint

Cell Death
Hot Temperature
Wounds and Injuries
Cellular Immunity
Blood Cells
Apoptosis
DNA Breaks
Agar Gel Electrophoresis
Propidium
Body Surface Area
Phytohemagglutinins
Phorbol Esters
DNA Fragmentation
Burns
Lectins
Interleukin-2
Cell Survival
Coloring Agents
Lymphocytes
Staining and Labeling

Keywords

  • apoptosis
  • Burns
  • immunodeficiency
  • T cell activation

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

Cite this

Immune deficiency following thermal trauma is associated with apoptotic cell death. / Teodorczyk-Injeyan, J. A.; Cembrzynska-Nowak, M.; Lalani, S.; Peters, W. J.; Mills, Gordon.

In: Journal of Clinical Immunology, Vol. 15, No. 6, 01.11.1995, p. 318-328.

Research output: Contribution to journalArticle

Teodorczyk-Injeyan, JA, Cembrzynska-Nowak, M, Lalani, S, Peters, WJ & Mills, G 1995, 'Immune deficiency following thermal trauma is associated with apoptotic cell death', Journal of Clinical Immunology, vol. 15, no. 6, pp. 318-328. https://doi.org/10.1007/BF01541322
Teodorczyk-Injeyan, J. A. ; Cembrzynska-Nowak, M. ; Lalani, S. ; Peters, W. J. ; Mills, Gordon. / Immune deficiency following thermal trauma is associated with apoptotic cell death. In: Journal of Clinical Immunology. 1995 ; Vol. 15, No. 6. pp. 318-328.
@article{b7a2eed380fe4c6095a532ef97a18265,
title = "Immune deficiency following thermal trauma is associated with apoptotic cell death",
abstract = "Thermal injury-associated specific immune deficiency occurs despite indicators of systemic activation of the lymphoid compartment. We investigated the possibility that postburn immune failure and T cell activation are causally related through activation-induced (apoptotic) cell death. The relationship between the cellular immune response and cell mortality was examined in cultures of peripheral blood mononuclear cells (PBMC) from 14 immunosuppressed patients with extensive burns (35-90{\%} total body surface area). Impaired cellular immunity coincided with significantly reduced cell viability as ascertained by propidium iodide staining and dye reduction assays. Following stimulation with the mitogenic lectin, phytohemagglutinin (PHA), the majority of DNA in patient cultures was fragmented, suggesting the occurrence of apoptotic cell death. Even without stimulation a portion of patient cells was apoptotic as indicated by oligonucleosomal bands on agarose gel electrophoresis. Exogenous interleukin-2 or phorbol ester markedly reduced constitutive as well as PHAinduced DNA fragmentation. In situ demonstration of DNA strand breaks in freshly isolated patient PBMC, by a TdT-based labeling technique, confirmed that a larger fraction (up to 60{\%}) of circulating lymphocytes was undergoing apoptosis on the periphery. These novel observations suggest that apoptosis may play a major role in thermal injury-related cellular immunodeficiency.",
keywords = "apoptosis, Burns, immunodeficiency, T cell activation",
author = "Teodorczyk-Injeyan, {J. A.} and M. Cembrzynska-Nowak and S. Lalani and Peters, {W. J.} and Gordon Mills",
year = "1995",
month = "11",
day = "1",
doi = "10.1007/BF01541322",
language = "English (US)",
volume = "15",
pages = "318--328",
journal = "Journal of Clinical Immunology",
issn = "0271-9142",
publisher = "Springer New York",
number = "6",

}

TY - JOUR

T1 - Immune deficiency following thermal trauma is associated with apoptotic cell death

AU - Teodorczyk-Injeyan, J. A.

AU - Cembrzynska-Nowak, M.

AU - Lalani, S.

AU - Peters, W. J.

AU - Mills, Gordon

PY - 1995/11/1

Y1 - 1995/11/1

N2 - Thermal injury-associated specific immune deficiency occurs despite indicators of systemic activation of the lymphoid compartment. We investigated the possibility that postburn immune failure and T cell activation are causally related through activation-induced (apoptotic) cell death. The relationship between the cellular immune response and cell mortality was examined in cultures of peripheral blood mononuclear cells (PBMC) from 14 immunosuppressed patients with extensive burns (35-90% total body surface area). Impaired cellular immunity coincided with significantly reduced cell viability as ascertained by propidium iodide staining and dye reduction assays. Following stimulation with the mitogenic lectin, phytohemagglutinin (PHA), the majority of DNA in patient cultures was fragmented, suggesting the occurrence of apoptotic cell death. Even without stimulation a portion of patient cells was apoptotic as indicated by oligonucleosomal bands on agarose gel electrophoresis. Exogenous interleukin-2 or phorbol ester markedly reduced constitutive as well as PHAinduced DNA fragmentation. In situ demonstration of DNA strand breaks in freshly isolated patient PBMC, by a TdT-based labeling technique, confirmed that a larger fraction (up to 60%) of circulating lymphocytes was undergoing apoptosis on the periphery. These novel observations suggest that apoptosis may play a major role in thermal injury-related cellular immunodeficiency.

AB - Thermal injury-associated specific immune deficiency occurs despite indicators of systemic activation of the lymphoid compartment. We investigated the possibility that postburn immune failure and T cell activation are causally related through activation-induced (apoptotic) cell death. The relationship between the cellular immune response and cell mortality was examined in cultures of peripheral blood mononuclear cells (PBMC) from 14 immunosuppressed patients with extensive burns (35-90% total body surface area). Impaired cellular immunity coincided with significantly reduced cell viability as ascertained by propidium iodide staining and dye reduction assays. Following stimulation with the mitogenic lectin, phytohemagglutinin (PHA), the majority of DNA in patient cultures was fragmented, suggesting the occurrence of apoptotic cell death. Even without stimulation a portion of patient cells was apoptotic as indicated by oligonucleosomal bands on agarose gel electrophoresis. Exogenous interleukin-2 or phorbol ester markedly reduced constitutive as well as PHAinduced DNA fragmentation. In situ demonstration of DNA strand breaks in freshly isolated patient PBMC, by a TdT-based labeling technique, confirmed that a larger fraction (up to 60%) of circulating lymphocytes was undergoing apoptosis on the periphery. These novel observations suggest that apoptosis may play a major role in thermal injury-related cellular immunodeficiency.

KW - apoptosis

KW - Burns

KW - immunodeficiency

KW - T cell activation

UR - http://www.scopus.com/inward/record.url?scp=0028839459&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0028839459&partnerID=8YFLogxK

U2 - 10.1007/BF01541322

DO - 10.1007/BF01541322

M3 - Article

C2 - 8576318

AN - SCOPUS:0028839459

VL - 15

SP - 318

EP - 328

JO - Journal of Clinical Immunology

JF - Journal of Clinical Immunology

SN - 0271-9142

IS - 6

ER -