Immortalization of Werner syndrome and progeria fibroblasts

Hiroshi Saito, Robb E. Moses

Research output: Contribution to journalArticle

47 Scopus citations

Abstract

Human fibroblast cells from two different progeroid syndromes, Werner syndrome (WS) and progeria, were established as immortalized cell lines by transfection with plasmid DNA containing the SV40 early region. The lineage of each immortalized cell line was confirmed by VNTR analysis. Each of the immortalized cell lines maintained its original phenotype of slow growth. DNA repair ability of these cells was also studied by measuring sensitivity to killing by uv or the DNA-damaging drugs methyl methansulfonate, bleomycin, and cis-dichlorodiamine platinum. The results showed that both WS and progeria cells have normal sensitivity to these agents.

Original languageEnglish (US)
Pages (from-to)373-379
Number of pages7
JournalExperimental Cell Research
Volume192
Issue number2
DOIs
StatePublished - Feb 1991

ASJC Scopus subject areas

  • Cell Biology

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