Immortalization of Werner syndrome and progeria fibroblasts

Hiroshi Saito; Robb E. Moses

doi:10.1016/0014-4827(91)90054-X

Immortalization of Werner syndrome and progeria fibroblasts

Hiroshi Saito, Robb E. Moses

Research output: Contribution to journal › Article › peer-review

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Abstract

Human fibroblast cells from two different progeroid syndromes, Werner syndrome (WS) and progeria, were established as immortalized cell lines by transfection with plasmid DNA containing the SV40 early region. The lineage of each immortalized cell line was confirmed by VNTR analysis. Each of the immortalized cell lines maintained its original phenotype of slow growth. DNA repair ability of these cells was also studied by measuring sensitivity to killing by uv or the DNA-damaging drugs methyl methansulfonate, bleomycin, and cis-dichlorodiamine platinum. The results showed that both WS and progeria cells have normal sensitivity to these agents.

Original language	English (US)
Pages (from-to)	373-379
Number of pages	7
Journal	Experimental Cell Research
Volume	192
Issue number	2
DOIs	https://doi.org/10.1016/0014-4827(91)90054-X
State	Published - Feb 1991
Externally published	Yes

ASJC Scopus subject areas

Cell Biology

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title = "Immortalization of Werner syndrome and progeria fibroblasts",

abstract = "Human fibroblast cells from two different progeroid syndromes, Werner syndrome (WS) and progeria, were established as immortalized cell lines by transfection with plasmid DNA containing the SV40 early region. The lineage of each immortalized cell line was confirmed by VNTR analysis. Each of the immortalized cell lines maintained its original phenotype of slow growth. DNA repair ability of these cells was also studied by measuring sensitivity to killing by uv or the DNA-damaging drugs methyl methansulfonate, bleomycin, and cis-dichlorodiamine platinum. The results showed that both WS and progeria cells have normal sensitivity to these agents.",

author = "Hiroshi Saito and Moses, {Robb E.}",

note = "Funding Information: This study was supported by USPHS Grant AG07123, a grant from the Fanconi Anemia Research Foundation, and a grant from the Sandoz Foundation for Gerontological Research.",

year = "1991",

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doi = "10.1016/0014-4827(91)90054-X",

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journal = "Experimental Cell Research",

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T1 - Immortalization of Werner syndrome and progeria fibroblasts

AU - Saito, Hiroshi

AU - Moses, Robb E.

N1 - Funding Information: This study was supported by USPHS Grant AG07123, a grant from the Fanconi Anemia Research Foundation, and a grant from the Sandoz Foundation for Gerontological Research.

PY - 1991/2

Y1 - 1991/2

N2 - Human fibroblast cells from two different progeroid syndromes, Werner syndrome (WS) and progeria, were established as immortalized cell lines by transfection with plasmid DNA containing the SV40 early region. The lineage of each immortalized cell line was confirmed by VNTR analysis. Each of the immortalized cell lines maintained its original phenotype of slow growth. DNA repair ability of these cells was also studied by measuring sensitivity to killing by uv or the DNA-damaging drugs methyl methansulfonate, bleomycin, and cis-dichlorodiamine platinum. The results showed that both WS and progeria cells have normal sensitivity to these agents.

AB - Human fibroblast cells from two different progeroid syndromes, Werner syndrome (WS) and progeria, were established as immortalized cell lines by transfection with plasmid DNA containing the SV40 early region. The lineage of each immortalized cell line was confirmed by VNTR analysis. Each of the immortalized cell lines maintained its original phenotype of slow growth. DNA repair ability of these cells was also studied by measuring sensitivity to killing by uv or the DNA-damaging drugs methyl methansulfonate, bleomycin, and cis-dichlorodiamine platinum. The results showed that both WS and progeria cells have normal sensitivity to these agents.

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Immortalization of Werner syndrome and progeria fibroblasts

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