Immortalization of four new fanconi anemia fibroblast cell lines by an improved procedure

P. M. Jakobs, P. Sahaayaruban, H. Saito, C. Reifsteck, S. Olson, H. Joenje, R. E. Moses, M. Grompe

Research output: Contribution to journalArticle

24 Scopus citations

Abstract

Fanconi anemia (FA) is an autosomal recessive disease characterized by birth defects, progressive bone marrow failure and increased risk for leukemia. FA cells display chromosome breakage and increased cell killing in response to DNA crosslinking agents. At least 5 genes have been defined by cell complementation studies, but only one of these, FAC has been cloned to date. Efforts to map and isolate new FA genes by functional complementation have been hampered by the lack of immortalized FA fibroblast cell lines. Here we report the use of a novel immortalization strategy to create 4 new immortalized FA fibroblast lines, including one from the rare complementation group D.

Original languageEnglish (US)
Pages (from-to)151-157
Number of pages7
JournalSomatic Cell and Molecular Genetics
Volume22
Issue number2
DOIs
StatePublished - Mar 1996

ASJC Scopus subject areas

  • Genetics
  • Cell Biology

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