Imaging pericytes and capillary diameter in brain slices and isolated retinae

Anusha Mishra, Fergus M. O'Farrell, Clare Reynell, Nicola B. Hamilton, Catherine N. Hall, David Attwell

Research output: Contribution to journalArticle

37 Citations (Scopus)

Abstract

The cerebral circulation is highly specialized, both structurally and functionally, and it provides a fine-tuned supply of oxygen and nutrients to active regions of the brain. Our understanding of blood flow regulation by cerebral arterioles has evolved rapidly. Recent work has opened new avenues in microvascular research; for example, it has been demonstrated that contractile pericytes found on capillary walls induce capillary diameter changes in response to neurotransmitters, suggesting that pericytes could have a role in neurovascular coupling. This concept is at odds with traditional models of brain blood flow regulation, which assume that only arterioles control cerebral blood flow. The investigation of mechanisms underlying neurovascular coupling at the capillary level requires a range of approaches, which involve unique technical challenges. Here we provide detailed protocols for the successful physiological and immunohistochemical study of pericytes and capillaries in brain slices and isolated retinae, allowing investigators to probe the role of capillaries in neurovascular coupling. This protocol can be completed within 6-8 h; however, immunohistochemical experiments may take 3-6 d.

Original languageEnglish (US)
Pages (from-to)323-336
Number of pages14
JournalNature Protocols
Volume9
Issue number2
DOIs
StatePublished - Feb 2014
Externally publishedYes

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Pericytes
Cerebrovascular Circulation
Retina
Brain
Blood
Imaging techniques
Arterioles
Nutrients
Neurotransmitter Agents
Oxygen
Research Personnel
Food
Experiments
Research
Neurovascular Coupling

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Mishra, A., O'Farrell, F. M., Reynell, C., Hamilton, N. B., Hall, C. N., & Attwell, D. (2014). Imaging pericytes and capillary diameter in brain slices and isolated retinae. Nature Protocols, 9(2), 323-336. https://doi.org/10.1038/nprot.2014.019

Imaging pericytes and capillary diameter in brain slices and isolated retinae. / Mishra, Anusha; O'Farrell, Fergus M.; Reynell, Clare; Hamilton, Nicola B.; Hall, Catherine N.; Attwell, David.

In: Nature Protocols, Vol. 9, No. 2, 02.2014, p. 323-336.

Research output: Contribution to journalArticle

Mishra, A, O'Farrell, FM, Reynell, C, Hamilton, NB, Hall, CN & Attwell, D 2014, 'Imaging pericytes and capillary diameter in brain slices and isolated retinae', Nature Protocols, vol. 9, no. 2, pp. 323-336. https://doi.org/10.1038/nprot.2014.019
Mishra, Anusha ; O'Farrell, Fergus M. ; Reynell, Clare ; Hamilton, Nicola B. ; Hall, Catherine N. ; Attwell, David. / Imaging pericytes and capillary diameter in brain slices and isolated retinae. In: Nature Protocols. 2014 ; Vol. 9, No. 2. pp. 323-336.
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