Imaging of glioma tumor with endogenous fluorescence tomography

Dax S. Kepshire, Summer L. Gibbs-Struass, Julia A. O'Hara, Michael Hutchins, Niculae Mincu, Frederic Leblond, Mario Khayat, Hamid Dehghani, Subhadra Srinivasan, Brian W. Pogue

Research output: Contribution to journalArticle

31 Scopus citations

Abstract

Tomographic imaging of a glioma tumor with endogenous fluorescence is demonstrated using a non-contact single-photon counting fan-beam acquisition system interfaced with microCT imaging. The fluorescence from protoporphyrin IX (PpIX) was found to be detectable, and allowed imaging of the tumor from within the cranium, even though the tumor presence was not visible in the microCT image. The combination of single-photon counting detection and normalized fluorescence to transmission detection at each channel allowed robust imaging of the signal. This demonstrated use of endogenous fluorescence stimulation from aminolevulinic acid (ALA) and provides the first in vivo demonstration of deep tissue tomographic imaging with protoporphyrin IX.

Original languageEnglish (US)
Article number030501
JournalJournal of biomedical optics
Volume14
Issue number3
DOIs
StatePublished - Jun 5 2009
Externally publishedYes

Keywords

  • Flurescence
  • Flurescence molecular tomography
  • Tomography

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Biomaterials
  • Atomic and Molecular Physics, and Optics
  • Biomedical Engineering

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    Kepshire, D. S., Gibbs-Struass, S. L., O'Hara, J. A., Hutchins, M., Mincu, N., Leblond, F., Khayat, M., Dehghani, H., Srinivasan, S., & Pogue, B. W. (2009). Imaging of glioma tumor with endogenous fluorescence tomography. Journal of biomedical optics, 14(3), [030501]. https://doi.org/10.1117/1.3127202