@inbook{2ba5e6232da74c3585c2613225b2fe42,
title = "Imaging H2O2 microdomains in receptor tyrosine kinases signaling",
abstract = "HyPer, a ratiometric genetically encoded fluorescent sensor, is a popular tool for intracellular hydrogen peroxide detection. When expressed in cultured cells, the freely diffusing version of the sensor (HyPer-cyto) detects temporal patterns of H2O2 generation. However, rapid diffusion of the probe within the nucleocytoplasmic compartment averages the H 2O2 signal even in cases of local oxidant production. Consequently, we immobilized the sensor within specific subcellular compartments allowing it to monitor local increases in H2O2. Here, we provide a protocol of ratiometric imaging and ImageJ-based quantification of H2O2 microdomains produced by cells upon physiological stimulation.",
keywords = "HyPer, Hydrogen peroxide microdomains, Imaging, NADPH oxidases, Receptor tyrosine kinases, Sensor, Signaling",
author = "Mishina, {Nataliya M.} and Markvicheva, {Kseniya N.} and Fradkov, {Arkady F.} and Zagaynova, {Elena V.} and Carsten Schultz and Sergey Lukyanov and Belousov, {Vsevolod V.}",
note = "Funding Information: This work was supported by the Russian foundation for basic research (RFBR) (10-04-01561-a, V. V. B.; 11-04-12187-ofi, S. L.), a joint EMBL-RFBR grant (12-04-92427 to C. S. and V. V. B.), Measures to Attract Leading Scientists to Russian Educational Institutions program (11.G34.31.0017 to S. L.), and the ESF EuroMembrane consortium TraPPs (to C. S.). ",
year = "2013",
doi = "10.1016/B978-0-12-405883-5.00011-9",
language = "English (US)",
isbn = "9780124058835",
series = "Methods in Enzymology",
publisher = "Academic Press Inc.",
pages = "175--187",
booktitle = "Hydrogen Peroxide and Cell Signaling, Part A",
}