Imaging axonal transport in the rat visual pathway

Carla J. Abbott, Tiffany E. Choe, Theresa A. Lusardi, Claude F. Burgoyne, Lin Wang, Brad Fortune

Research output: Contribution to journalArticlepeer-review

27 Scopus citations

Abstract

A technique was developed for assaying axonal transport in retinal ganglion cells using 2 μl injections of 1% cholera toxin b-subunit conjugated to AlexaFluor488 (CTB). In vivo retinal and post-mortem brain imaging by confocal scanning laser ophthalmoscopy and post-mortem microscopy were performed. The transport of CTB was sensitive to colchicine, which disrupts axonal microtubules. The bulk rates of transport were determined to be approximately 80-90 mm/day (anterograde) and 160 mm/day (retrograde). Results demonstrate that axonal transport of CTB can be monitored in vivo in the rodent anterior visual pathway, is dependent on intact microtubules, and occurs by active transport mechanisms.

Original languageEnglish (US)
Pages (from-to)364-386
Number of pages23
JournalBiomedical Optics Express
Volume4
Issue number2
DOIs
StatePublished - Feb 2013

ASJC Scopus subject areas

  • Biotechnology
  • Atomic and Molecular Physics, and Optics

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