Imaging actin and dynamin recruitment during invagination of single clathrin-coated pits

Christen J. Merrifield, Morris E. Feldman, Lei Wan, Wolfhard Almers

Research output: Contribution to journalArticle

516 Citations (Scopus)

Abstract

As a final step in endocytosis, clathrin-coated pits must separate from the plasma membrane and move into the cytosol as a coated vesicle. Because these events involve minute movements that conventional light microscopy cannot resolve, they have not been observed directly and their dynamics remain unexplored. Here, we used evanescent field (EF) microscopy to observe single clathrin-coated pits or vesicles as they draw inwards from the plasma membrane and finally lose their coats. This inward movement occurred immediately after a brief burst of dynamin recruitment and was accompanied by transient actin assembly. Therefore, dynamin may provide the trigger and actin may provide the force for movement into the cytosol.

Original languageEnglish (US)
Pages (from-to)691-698
Number of pages8
JournalNature Cell Biology
Volume4
Issue number9
DOIs
StatePublished - Sep 2002

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Dynamins
Clathrin
Cytosol
Actins
Microscopy
Cell Membrane
Coated Vesicles
Endocytosis
Light

ASJC Scopus subject areas

  • Cell Biology

Cite this

Imaging actin and dynamin recruitment during invagination of single clathrin-coated pits. / Merrifield, Christen J.; Feldman, Morris E.; Wan, Lei; Almers, Wolfhard.

In: Nature Cell Biology, Vol. 4, No. 9, 09.2002, p. 691-698.

Research output: Contribution to journalArticle

Merrifield, Christen J. ; Feldman, Morris E. ; Wan, Lei ; Almers, Wolfhard. / Imaging actin and dynamin recruitment during invagination of single clathrin-coated pits. In: Nature Cell Biology. 2002 ; Vol. 4, No. 9. pp. 691-698.
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